Comparison of a Semiautomated Commercial Repetitive-Sequence-Based PCR Method with Spoligotyping, 24-Locus Mycobacterial Interspersed Repetitive-Unit–Variable-Number Tandem-Repeat Typing, and Restriction Fragment Length Polymorphism-Based Analysis of IS6110 for Mycobacterium tuberculosis Typing

F. Brossier; C. Sola; G. Millot; V. Jarlier; N. Veziris; W. Sougakoff

首页> 外文期刊>Journal of Clinical Microbiology >Comparison of a Semiautomated Commercial Repetitive-Sequence-Based PCR Method with Spoligotyping, 24-Locus Mycobacterial Interspersed Repetitive-Unit–Variable-Number Tandem-Repeat Typing, and Restriction Fragment Length Polymorphism-Based Analysis of IS6110 for Mycobacterium tuberculosis Typing

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Comparison of a Semiautomated Commercial Repetitive-Sequence-Based PCR Method with Spoligotyping, 24-Locus Mycobacterial Interspersed Repetitive-Unit–Variable-Number Tandem-Repeat Typing, and Restriction Fragment Length Polymorphism-Based Analysis of IS6110 for Mycobacterium tuberculosis Typing

机译：半自动商业重复序列PCR方法与Spoligotyping，24位点分枝杆菌重复单元-可变数串联重复序列分型和基于限制性片段长度多态性的IS6110结核分枝杆菌分型分析的比较

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Fifty-two multidrug-resistant isolates of Mycobacterium tuberculosis representative of the currently predominant lineages in France were analyzed using repetitive-sequence-based PCR (rep-PCR) DiversiLab (DL), spoligotyping, 24-locus mycobacterial interspersed repetitive-unit–variable-number tandem-repeat typing (MIRU-VNTR), and restriction fragment length polymorphism of IS6110 (IS6110-RFLP). DL, as opposed to MIRU-VNTR and IS6110-RFLP analysis, did not allow discrimination among half of the isolates, an indication of comparatively lower resolving power.

机译：使用基于重复序列的PCR（rep-PCR）DiversiLab（DL），刻板分型，24位分枝杆菌散布的重复单元可变变量，分析了法国目前主要血统的52株结核分枝杆菌的多药耐药菌株IS 6110 （IS 6110 -RFLP）的数量串联重复型（MIRU-VNTR）和限制性片段长度多态性。与MIRU-VNTR和IS 6110 -RFLP分析相反，DL不能区分一半的分离株，这表明分离能力相对较低。 展开▼

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《Journal of Clinical Microbiology》 |2014年第11期|共5页

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F. Brossier; C. Sola; G. Millot; V. Jarlier; N. Veziris; W. Sougakoff;
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中图分类医学微生物学（病原细菌学、病原微生物学）;

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专利

1. Use of Variable-Number Tandem-Repeat Typing To Differentiate Mycobacterium tuberculosis Beijing Family Isolates from Hong Kong and Comparison with IS6110 Restriction Fragment Length Polymorphism Typing and Spoligotyping [J] . Kristin Kremer, Betty Kam Yan Au, Peter Chi Wai Yip, Journal of Clinical Microbiology . 2005,第1期

机译：利用可变数目串联重复分型区分香港的北京分枝杆菌和与IS6110限制性片段长度多态性分型和寡核苷酸分型的比较

2. Discordance between Mycobacterial Interspersed Repetitive-Unit-Variable-Number Tandem-Repeat Typing and IS6110 Restriction Fragment Length Polymorphism Genotyping for Analysis of Mycobacterium tuberculosis Beijing Strains in a Setting of High Incidence of Tuberculosis [J] . M. Hanekom, G. D. van der Spuy, N. C. Gey van Pittius, Journal of Clinical Microbiology . 2008,第10期

机译：在结核高发地区分析结核分枝杆菌北京菌株的分枝杆菌散布的重复单位可变数目串联重复序列分型和IS6110限制性片段长度多态性基因分型

3. Comparison of a Variable-Number Tandem-Repeat (VNTR) Method for Typing Mycobacterium avium with Mycobacterial Interspersed Repetitive-Unit-VNTR and IS1245 Restriction Fragment Length Polymorphism Typing [J] . Takayuki Inagaki, Kei Nishimori, Tetsuya Yagi, Journal of Clinical Microbiology . 2009,第7期

机译：可变数目串联重复（VNTR）方法分型鸟分枝杆菌与分枝杆菌散布的重复单元-VNTR和IS1245限制性片段长度多态性分型的比较。

4. Parallel application of Variable-Number Tandem-Repeat Markers typing and IS 1245 Restriction Fragment Length Polymorphism fingerprinting of Finnish Mycobacterium avium from human and porcine origins [C] . Tirkkonen Taneli, Pakarinen Jaakko, Rintala Elina, Congress of the International Pig Veterinary Society . 2008

机译：通过人和猪起源于人和猪起源，可变数量串联重复标记键入和是1245个限制片段长度多态性指纹印刷。来自人和猪起源的芬兰分枝杆菌

5. Comparison of a Semiautomated Commercial Repetitive-Sequence-Based PCR Method with Spoligotyping 24-Locus Mycobacterial Interspersed Repetitive-Unit–Variable-Number Tandem-Repeat Typing and Restriction Fragment Length Polymorphism-Based Analysis of IS6110 for Mycobacterium tuberculosis Typing [O] . F. Brossier, C. Sola, G. Millot, 2014

机译：半自动化的基于商业重复序列的PCR方法与寡核苷酸分型24位点分枝杆菌重复单元-可变数串联重复序列分型和基于限制性片段长度多态性的IS6110结核分枝杆菌分型分析的比较

6. Complexity of DRw6 DR5 Haplotypes in American Blacks Demonstrated by Serology, Cellular Typing, and Restriction Fragment Length Polymorphism Analysis. [R] . Lee, K. W., Hurley, C. K., Hartzman, R., 1990

机译：美国黑人DRw6 DR5单倍型的复杂性通过血清学，细胞分型和限制性片段长度多态性分析得到证实。

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2. 多位点可变数量串联重复序列分析在北京家族结核分枝杆菌基因分型中的应用及位点筛选 [J] . 郑晓静 ,郑素华 ,杜博平 . 临床肺科杂志 . 2010,第007期

3. 肠炎沙门菌可变数目串联重复序列位点用于多位点可变数目串联重复序列分型分析的评价 [J] . 张力 ,韩辉 ,周海健 . 中华预防医学杂志 . 2011,第006期

4. 福建省结核分枝杆菌多位点可变数目串联重复序列基因分型研究 [J] . 梁庆福 ,赵雁林 ,陈求扬 . 中华流行病学杂志 . 2012,第011期

5. 结核分枝杆菌多位点可变数目串联重复序列分型方法标准化操作程序的建立 [J] . 吕冰 ,Christine Pourcel ,刘敬华 . 中华流行病学杂志 . 2008,第009期

6. 不同可变数目串联重复序列组合对中国流行结核分枝杆菌分辨力的评价研究 [C] . 陈海霞 ,刘静仪 ,张治国 . 中国防痨协会第32届全国学术大会 . 2018

7. 基于可变数目串联重复序列的痰液结核分枝杆菌检测方法建立及初步应用 [A] . 苏敏 . 2016

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2. METHOD OF GENETIC TYPING OF YERSINIA PESTIS AND YERSINIA PSEUDOTUBERCULOSIS BASED ON THE ANALYSIS OF 13 VNTR LOCUSES IN MULTIPLEX PCR REACTION [P] . 外国专利： RU2644236C1 . 2018-02-08

机译：基于多重PCR反应中13个VNTR位点分析的鼠疫耶尔森菌和假结核耶尔森氏菌基因分型方法

3. Polymerase chain reaction/restriction fragment length polymorphism method for the detection and typing of myobacteria [P] . 外国专利： US5543294A . 1996-08-06

机译：聚合酶链反应/限制性片段长度多态性检测及分型的方法

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Comparison of a Semiautomated Commercial Repetitive-Sequence-Based PCR Method with Spoligotyping, 24-Locus Mycobacterial Interspersed Repetitive-Unit–Variable-Number Tandem-Repeat Typing, and Restriction Fragment Length Polymorphism-Based Analysis of IS6110 for Mycobacterium tuberculosis Typing

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