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首页> 外文期刊>Journal of Clinical Microbiology >Use of ChromID Extended-Spectrum β-Lactamase Medium for Detecting Carbapenemase-Producing Enterobacteriaceae
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Use of ChromID Extended-Spectrum β-Lactamase Medium for Detecting Carbapenemase-Producing Enterobacteriaceae

机译:使用ChromID广谱β-内酰胺酶培养基检测产碳青霉烯酶的肠杆菌科

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ChromID extended-spectrum β-lactamase (ESBL) culture medium is routinely used for screening ESBL producers. This medium was tested for detecting carbapenemase-producing Enterobacteriaceae isolates from a collection of reference strains and compared to the CHROMagar KPC culture medium previously evaluated for detecting KPC-producing isolates. Producers of IMP-, VIM-, and KPC-type carbapenemases with high levels of resistance to cephalosporins and to carbapenems were detected at 1 × 101 CFU/ml. The OXA-48 producers were not detected on ChromID ESBL medium unless coexpressing ESBLs, whereas carbapenemase-producing isolates with MICs of <4 μg/ml were not detected on CHROMagar KPC medium.
机译:ChromID广谱β-内酰胺酶(ESBL)培养基通常用于筛选ESBL生产者。测试了该培养基以检测来自一系列参考菌株的产生碳青霉烯酶的肠杆菌科细菌的分离株,并与先前评估用于检测产生KPC的菌株的CHROMagar KPC培养基进行了比较。以1×10 1 CFU / ml检测到具有高水平的对头孢菌素和碳青霉烯类耐药性的IMP-,VIM-和KPC型碳青霉烯酶的生产者。除非共表达ESBL,否则在ChromID ESBL培养基上未检测到OXA-48生产者,而在CHROMagar KPC培养基上未检测到MIC小于4μg/ ml的碳青霉烯酶生产菌株。

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