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首页> 外文期刊>Journal of Clinical Microbiology >Analysis of the Bacterial Communities Present in Lungs of Patients with Cystic Fibrosis from American and British Centers
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Analysis of the Bacterial Communities Present in Lungs of Patients with Cystic Fibrosis from American and British Centers

机译：来自美国和英国中心的囊性纤维化患者肺部细菌群落分析

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The aim of this study was to determine whether geographical differences impact the composition of bacterial communities present in the airways of cystic fibrosis (CF) patients attending CF centers in the United States or United Kingdom. Thirty-eight patients were matched on the basis of clinical parameters into 19 pairs comprised of one U.S. and one United Kingdom patient. Analysis was performed to determine what, if any, bacterial correlates could be identified. Two culture-independent strategies were used: terminal restriction fragment length polymorphism (T-RFLP) profiling and 16S rRNA clone sequencing. Overall, 73 different terminal restriction fragment lengths were detected, ranging from 2 to 10 for U.S. and 2 to 15 for United Kingdom patients. The statistical analysis of T-RFLP data indicated that patient pairing was successful and revealed substantial transatlantic similarities in the bacterial communities. A small number of bands was present in the vast majority of patients in both locations, indicating that these are species common to the CF lung. Clone sequence analysis also revealed that a number of species not traditionally associated with the CF lung were present in both sample groups. The species number per sample was similar, but differences in species presence were observed between sample groups. Cluster analysis revealed geographical differences in bacterial presence and relative species abundance. Overall, the U.S. samples showed tighter clustering with each other compared to that of United Kingdom samples, which may reflect the lower diversity detected in the U.S. sample group. The impact of cross-infection and biogeography is considered, and the implications for treating CF lung infections also are discussed.
机译：这项研究的目的是确定地理差异是否会影响到在美国或英国的CF中心就诊的囊性纤维化（CF）患者气道中存在的细菌群落组成。根据临床参数将38例患者分为19对，包括1名美国患者和1名英国患者。进行分析以确定可以鉴定出哪些细菌相关性。使用了两种与文化无关的策略：末端限制性片段长度多态性（T-RFLP）分析和16S rRNA克隆测序。总体上，检测到73种不同的末端限制性片段长度，美国的范围为2到10，英国患者的范围​​为2到15。 T-RFLP数据的统计分析表明，患者配对是成功的，并且揭示了细菌群落中的跨大西洋相似之处。在这两个位置的绝大部分患者中均存在少量条带，表明这些条带是CF肺常见的物种。克隆序列分析还显示，两个样本组中都存在许多传统上与CF肺不相关的物种。每个样品的物种数相似，但是在样品组之间观察到物种存在的差异。聚类分析显示细菌存在和相对物种丰度的地理差异。总体而言，与英国样品相比，美国样品显示出更紧密的聚类，这可能反映了美国样品组中检测到的多样性较低。考虑了交叉感染和生物地理学的影响，并讨论了治疗CF肺部感染的意义。

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