...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Comparison of the Luminex xTAG RVP Fast Assay and the Idaho Technology FilmArray RP Assay for Detection of Respiratory Viruses in Pediatric Patients at a Cancer Hospital
【24h】

Comparison of the Luminex xTAG RVP Fast Assay and the Idaho Technology FilmArray RP Assay for Detection of Respiratory Viruses in Pediatric Patients at a Cancer Hospital

机译：Luminex xTAG RVP快速测定法与爱达荷州Filmfilm RP测定法在癌症医院儿科患者中检测呼吸道病毒的比较

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Respiratory viruses are increasingly recognized as serious causes of morbidity and mortality in immunocompromised patients. The rapid and sensitive detection of respiratory viruses is essential for the early diagnosis and administration of appropriate antiviral therapy, as well as for the effective implementation of infection control measures. We compared the performance of two commercial assays, xTAG RVP Fast (Luminex Diagnostics, Toronto, Canada) and FilmArray RVP (FA RVP; Idaho Technology, Salt Lake City, UT), in pediatric patients at Memorial Sloan-Kettering Cancer Center. These assays detect the following viruses: respiratory syncytial virus; influenza A and B viruses; parainfluenza viruses 1, 2, 3, and 4; human metapneumovirus; adenovirus; enterovirus-rhinovirus; coronaviruses NL63, HKU1, 229E, and OC43; and bocavirus. We tested a total of 358 respiratory specimens from 173 pediatric patients previously tested by direct fluorescence assay (DFA) and viral culture. The overall detection rate (number of positive specimens/total specimens) for viruses tested by all methods was 24% for DFA/culture, 45% for xTAG RVP Fast, and 51% for FA RVP. The agreement between the two multiplex assays was 84.5%, and the difference in detection rate was statistically significant (P < 0.0001). Overall, the FA RVP assay was more sensitive than the xTAG RVP Fast assay and had a turnaround time of approximately 1 h. The sensitivity, simplicity, and random-access platform make FA RVP an excellent choice for laboratory on-demand service with low to medium volume.
机译：呼吸道病毒越来越被认为是免疫功能低下患者发病和死亡的严重原因。快速，灵敏地检测呼吸道病毒对于早期诊断和给予适当的抗病毒治疗以及有效实施感染控制措施至关重要。我们在纪念斯隆-凯特琳癌症中心的儿科患者中比较了两种商业化检测xTAG RVP Fast（Luminex Diagnostics，加拿大多伦多）和FilmArray RVP（FA RVP;爱达荷技术公司，犹他州盐湖城）的性能。这些检测可检测以下病毒：呼吸道合胞病毒;甲型和乙型流感病毒;副流感病毒1、2、3和4;人间质肺病毒;腺病毒肠病毒-鼻病毒冠状病毒NL63，HKU1、229E和OC43;和博卡病毒。我们测试了173名儿科患者的358个呼吸道标本，这些标本以前通过直接荧光测定（DFA）和病毒培养进行了测试。所有方法测试的病毒的总检出率（阳性标本数量/总标本数量）对于DFA /培养物为24％，对于xTAG RVP Fast为45％，对于FA RVP为51％。两次多重检测之间的一致性为84.5％，检出率的差异具有统计学意义（ P <0.0001）。总体而言，FA RVP测定比xTAG RVP快速测定更灵敏，周转时间约为1小时。 FA RVP的灵敏性，简单性和随机访问平台使其成为中小批量实验室按需服务的绝佳选择。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号