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首页> 外文期刊>Journal of Clinical Microbiology >Haemophilus influenzae ATCC 49766, an alternative quality control strain for monitoring broth microdilution susceptibility tests with selected beta-lactams.
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Haemophilus influenzae ATCC 49766, an alternative quality control strain for monitoring broth microdilution susceptibility tests with selected beta-lactams.

机译:流感嗜血杆菌ATCC 49766,一种替代质量控制菌株,用于监测使用选定的β-内酰胺进行的肉汤微稀释敏感性测试。

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A beta-lactamase-negative, ampicillin-resistant strain of Haemophilus influenzae is currently used for quality control of broth microdilution tests performed with Haemophilus test medium (HTM). Studies with eight lots of HTM broth documented the fact that MIC limits for some antimicrobial agents are unrealistically stringent; i.e., only three of eight lots of HTM broth were satisfactory for testing cefaclor. An alternative, ampicillin-susceptible strain of H. influenzae (ATCC 49766) was found to provide much more reproducible results with five problematic drugs (cefaclor, cefuroxime, cefamandole, loracarbef, and cefonicid). Multilaboratory studies defined MIC control limits for both control strains tested against 12 antimicrobial agents.
机译:流感嗜血杆菌的β-内酰胺酶阴性,耐氨苄西林的菌株目前用于通过嗜血杆菌测试培养基(HTM)进行的肉汤微量稀释测试的质量控制。对八批HTM肉汤的研究证明了以下事实:某些抗菌剂的MIC限制不切实际。也就是说,八批HTM肉汤中只有三批对头孢克洛测试合格。发现另一种氨苄青霉素敏感的流感嗜血杆菌菌株(ATCC 49766)与五种有问题的药物(头孢克洛,头孢呋辛,头孢曼多尔,洛拉卡培夫和头孢尼西德)可提供更高的重现性。多实验室研究确定了针对12种抗微生物剂测试的两种控制菌株的MIC控制极限。

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