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首页> 外文期刊>Journal of Clinical Microbiology >Genotyping of Mycoplasma pneumoniaeClinical Isolates Reveals Eight P1 Subtypes within Two Genomic Groups
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Genotyping of Mycoplasma pneumoniaeClinical Isolates Reveals Eight P1 Subtypes within Two Genomic Groups

机译：肺炎支原体的基因分型临床分离显示两个基因组中的八个P1亚型。

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Three methods for genotyping of Mycoplasma pneumoniaeclinical isolates were applied to 2 reference strains and 21 clinical isolates. By a modified restriction fragment length polymorphism (RFLP) analysis of PCR products of the M. pneumoniae cytadhesin P1 gene, 5 subtypes were discriminated among 13 P1 type 1 strains and 3 subtypes were discriminated among 8 P1 type 2 strains. Sequence analysis of the 16S-23S rRNA gene spacer region and part of the 23S rRNA gene revealed one nucleotide difference in the intergenic spacer region in 3 of the 21 isolates. In the 23S rRNA gene sequence of the 8 P1 type 2 strains an extra adenosine was present, but it was absent from the 13 P1 type 1 strains. On the basis of M. pneumoniae genome sequence data, primers were designed to amplify large interrepeat fragments by long PCR, and these fragments were subsequently analyzed by RFLP analysis. Only two types, long PCR types 1 and 2, could be discriminated among the M. pneumoniaeisolates. All P1 type 1 strains were assigned to long PCR type 1, and all P1 type 2 strains were assigned to long PCR type 2. These data obtained by three independent typing methods thus confirm the existence of two distinct M. pneumoniae genomic groups but expand the possibility of strain typing on the basis of variations within their P1 genes.
机译：将3种肺炎支原体临床分离株的基因分型方法应用于2株参考菌株和21株临床分离株。通过修饰的限制性片段长度多态性（RFLP）分析 M的PCR产物。肺炎性细胞粘附素P1基因在13个P1 1型菌株中有5个亚型，在8个P1 2型菌株中有3个亚型。对16S-23S rRNA基因间隔区和23S rRNA基因的一部分进行序列分析，发现21个分离物中的3个在基因间隔区中存在一个核苷酸差异。在8个P1 2型菌株的23S rRNA基因序列中，存在额外的腺苷，但在13个P1 1型菌株中不存在。根据 M。肺炎基因组序列数据，设计引物通过长PCR扩增大的重复片段，然后通过RFLP分析对这些片段进行分析。在 M中只能区分两种类型，即长PCR类型1和2。肺炎分离株。所有P1型1菌株均被指定为长PCR 1型，所有P1 2型菌株均被指定为长PCR 2型。通过三种独立的分型方法获得的这些数据证实了两个不同的 M的存在。肺炎基因组，但根据其P1基因内的变异扩大了菌株分型的可能性。

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