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首页> 外文期刊>Journal of Clinical Microbiology >Use of Random Amplified Polymorphic DNA PCR To Examine Epidemiology of Stenotrophomonas maltophilia andAchromobacter (Alcaligenes)xylosoxidans from Patients with Cystic Fibrosis
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Use of Random Amplified Polymorphic DNA PCR To Examine Epidemiology of Stenotrophomonas maltophilia andAchromobacter (Alcaligenes)xylosoxidans from Patients with Cystic Fibrosis

机译：利用随机扩增多态性DNA PCR检测囊性纤维化患者嗜麦芽窄食单胞菌和嗜色性无色杆菌木糖氧化酶的流行病学

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Stenotrophomonas maltophilia andAchromobacter (Alcaligenes)xylosoxidans have been increasingly recognized as a cause of respiratory tract colonization in cystic fibrosis (CF). Although both organisms have been associated with progressive deterioration of pulmonary function, demonstration of causality is lacking. To examine the molecular epidemiology of S. maltophilia and A. xylosoxidans in CF, isolates from patients monitored for up to 2 years were fingerprinted using a PCR-based randomly amplified polymorphic DNA (RAPD-PCR) method. Sixty-one of 69 CF centers screened had 183 S. maltophilia culture-positive patients, and 46 centers had 92A. xylosoxidans-positive patients. At least one isolate from each patient was genotyped, and patients with ≥10 positive cultures (12 S. maltophilia cultures, 15 A. xylosoxidans cultures) had serial isolates genotyped. In addition, centers with multiple culture-positive patients were examined for evidence of shared clones. There were no instances of shared genotypes among different CF centers. Some patients demonstrated isolates with a single genotype throughout the observation period, and others had intervening or sequential genotypes. At the six centers with multiple S. maltophilia culture-positive patients and the seven centers with multiple A. xylosoxidans-positive patients, there were three and five instances of shared genotypes, respectively. The majority of shared isolates were from pairs who were siblings or otherwise epidemiologically linked. These findings suggest RAPD-PCR typing can distinguish unique CF isolates of S. maltophilia and A. xylosoxidans, person-to-person transmission may occur, there are not a small number of clones infecting CF airways, and patients with long-term colonization may either have a persistent organism or may acquire additional organisms over time.
机译：嗜麦芽窄食单胞菌和无色杆菌（ alcaligenes ） xylosoxidans 被越来越多地认为是囊性纤维化中呼吸道定植的原因。 （CF）。尽管两种生物都与肺功能的逐步恶化有关，但缺乏因果关系的证明。检查 S的分子流行病学。嗜麦芽和 A。使用基于PCR的随机扩增多态性DNA（RAPD-PCR）方法对CF中长达2年的患者分离物中的木糖氧化酶进行了指纹识别。筛选出的69个CF中心中有61个具有183S。嗜麦芽细胞增多症阳性的患者，有46个中心的92 A。木糖氧化酶阳性患者。对每位患者的至少一个分离株进行基因分型，并且具有≥10个阳性培养物（12个S.em.maltophilia 培养物，15个 A。xylosoxidans 培养物）的患者具有连续分型。此外，检查了多个培养阳性患者的中心是否存在共享克隆的证据。在不同的CF中心之间没有共享基因型的实例。在整个观察期内，一些患者表现出具有单一基因型的分离株，而其他患者则具有中间或顺序的基因型。在六个具有多个 S的中心。嗜麦芽炎培养阳性患者和七个患有多个 A的中心。木聚糖氧化酶阳性患者，分别有3和5个共有基因型实例。大多数共享的分离株来自兄弟姐妹或在流行病学上有联系的对。这些发现表明RAPD-PCR分型可以区分 S的独特CF分离株。嗜麦芽和 A。木聚糖氧化酶可能会发生人与人之间的传播，并且不存在少数感染CF气道的克隆，长期定植的患者可能具有持久性生物体，或者随着时间的推移可能会获得其他生物体。

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