...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Molecular Analysis of a Hospital Cafeteria-Associated Salmonellosis Outbreak Using Modified Repetitive Element PCR Fingerprinting
【24h】

Molecular Analysis of a Hospital Cafeteria-Associated Salmonellosis Outbreak Using Modified Repetitive Element PCR Fingerprinting

机译：医院食堂相关沙门氏菌病暴发的分子分析，使用改进的重复元素PCR指纹图谱。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A hospital cafeteria-associated outbreak of gastroenteritis due toSalmonella enterica serotype Infantis was retrospectively evaluated using modified repetitive element PCR (rep-PCR) fingerprinting with the ERIC2 and BOXA1R primers and computer-assisted gel analysis and dendrogram construction. Rep-PCR yielded objective between-cycler, same-strain similarity values of from 92% (composite fingerprints) to 96% (ERIC2 fingerprints). The 70Salmonella isolates (which included 19 serotype Infantis isolates from the hospital outbreak, 10 other serotype Infantis isolates, and 41 isolates representing 14 other serotypes) were resolved well to the serotype level with each of the three fingerprint types (ERIC2, BOXA1R, and composite). Rep-PCR typing uncovered several historical serotyping errors and provided presumptive serotype assignments for other isolates with incomplete or undetermined serotypes. Analysis of replicate fingerprints for each isolate, as generated on two different thermal cyclers, indicated that most of the seeming subserotype discrimination noted in single-cycler dendrograms actually represented assay variability, since it was not reproducible in combined-cycler dendrograms. Rep-PCR typing, which would have been able to identify the presence of the hospital-associated serotype Infantis outbreak after the second outbreak isolate, could be used as a simple surrogate for serotyping by clinical microbiology laboratories that are equipped for diagnostic PCR.
机译：使用改良的重复元素PCR（rep-PCR）指纹图谱和ERIC2和BOXA1R引物，结合计算机辅助凝胶分析和树状图构建，回顾性评估了因食管炎小肠沙门氏菌血清型肠炎沙门氏菌引起的医院食堂相关性胃肠炎暴发。 Rep-PCR产生客观的循环之间，相同菌株相似性值，从92％（复合指纹）到96％（ERIC2指纹）。用三个指纹图谱将70株沙门氏菌分离株（其中包括医院暴发的19种血清型Infantis分离株，10种其他血清型Infantis分离株和41种代表其他14种血清型的分离株）均能很好地分离到血清型水平类型（ERIC2，BOXA1R和复合）。 Rep-PCR分型发现了几个历史血清分型错误，并为血清型不完全或不确定的其他分离株提供了推测的血清型分配。在两个不同的热循环仪上生成的每个分离株的重复指纹图谱的分析表明，单循环图谱中记录的大多数看似亚血清型的歧视实际上代表了测定变异性，因为在联合循环图谱中不可再现。 Rep-PCR分型能够确定第二次疫情爆发后医院相关血清型Infantis暴发的存在，可以被配备有诊断性PCR的临床微生物实验室用作血清分型的简单替代品。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号