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首页> 外文期刊>Journal of Clinical Microbiology >Phenotypic and Genotypic Characterization of Provisional Serotype Shigella flexneri 1c and Clonal Relationships with 1a and 1b Strains Isolated in Bangladesh
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Phenotypic and Genotypic Characterization of Provisional Serotype Shigella flexneri 1c and Clonal Relationships with 1a and 1b Strains Isolated in Bangladesh

机译：临时血清型弗氏志贺氏菌1c的表型和基因型特征以及与孟加拉国分离的1a和1b菌株的克隆关系

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The serotypes of 144 strains of Shigella flexneri serotype 1 (serotypes 1a, 1b, and 1c) isolated from patients attending the Dhaka treatment center of the International Centre for Diarrhoeal Disease Research, Bangladesh, between 1997 and 2001 were serologically confirmed by using commercially available antisera and a panel of monoclonal antibodies specific for S. flexneri group and type factor antigen (MASF). Among serotype 1 isolates, the prevalence of provisional serotype S. flexneri 1c increased from 0 to 56% from 1978 to 2001 in Bangladesh. Detailed biochemical studies revealed that none of the strains of serotype 1 produced indole, while all the strains fermented mannose, mannitol, and trehalose. Twenty percent of the serotype 1c and all the serotype 1a strains fermented maltose and 53% of the serotype 1c strains and 60% of the serotype 1a strains fermented arabinose, whereas all serotype 1b strains were negative for fermentation of these sugars. Only 18% of serotype 1b strains were resistant to nalidixic acid, and most of the serotype 1c and 1b strains were resistant to ampicillin, tetracycline, and trimethoprim-sulfamethoxazole. All the strains of serotypes 1a and 1b and about 88% of the serotype 1c strains were found to be invasive by the Sereny test, had a 140-MDa plasmid, and had Congo red absorption ability. Plasmid profile analysis showed that 26% of the strains of serotype 1 contained identical patterns. Most of the serotype 1c strains (72%) had the 1.6-MDa plasmid, which was not found in either serotype 1a or 1b strains. A self-transmissible middle-range plasmid (35 to 80 MDa) was found in some strains carrying the multiple-antibiotic-resistance gene. Pulsed-field gel electrophoresis analysis yielded three types (types A, B, and C) with numerous subtypes among the serotype 1c strains, whereas serotypes 1b and 1a yielded only one type for each serotype, and those types were related to the types for serotype 1c strains. Ribotyping analysis yielded three patterns for serotype 1c strains and one pattern each for serotype 1a and 1b strains which were similar to the patterns for the serotype 1c strains. Overall analysis of the results concluded that subserotype 1c is closely related to serotypes 1a and 1b. Furthermore, the high rate of prevalence of serotype 1c necessitates the commercial production of antibody against this subserotype to allow the determination of the actual burden of shigellosis caused by provisional serotype 1c.
机译：1997年至2001年间从孟加拉国国际腹泻病研究中心达卡治疗中心就诊的患者分离出的144株志贺氏菌血清型1（血清型1a，1b和1c）的血清型为通过使用可商购的抗血清和一组针对 S的单克隆抗体进行血清学确认。弗氏菌群和类型因子抗原（MASF）。在血清型1分离株中，临时血清型 S的流行。从1978年到2001年，孟加拉国的flexneri 1c从0％上升到56％。详细的生化研究表明，血清型1的菌株均未产生吲哚，而所有菌株均发酵了甘露糖，甘露醇和海藻糖。血清型1c和所有血清型1a菌株的20％发酵麦芽糖，血清型1c菌株的53％和血清型1a菌株的60％发酵阿拉伯糖，而所有血清型1b菌株对这些糖的发酵均呈阴性。仅18％的血清型1b菌株对萘啶酸有抗性，大多数血清型1c和1b菌株对氨苄青霉素，四环素和甲氧苄啶-磺胺甲恶唑有抗性。通过Sereny试验发现所有血清型1a和1b菌株以及约88％的血清型1c菌株都具有侵袭性，具有140-MDa质粒，并且具有刚果红吸收能力。质粒谱分析表明，血清型1的菌株中有26％包含相同的模式。大多数血清型1c菌株（72％）具有1.6-MDa质粒，在血清型1a或1b菌株中均未发现。在一些带有多重抗生素抗性基因的菌株中发现了一种自传中距离质粒（35至80 MDa）。脉冲场凝胶电泳分析产生了3种类型（A，B和C型），其中血清型1c菌株中有许多亚型，而血清型1b和1a每种血清型仅产生一种类型，这些类型与血清型有关1c株。核型分析产生了血清型1c菌株的三种模式，以及血清型1a和1b菌株的每种模式，与血清型1c菌株的模式相似。对结果的整体分析得出结论，亚型1c与1a和1b血清型密切相关。此外，血清型1c的高流行率使得必须商业生产针对该亚型的抗体，以测定由临时血清型1c引起的志贺菌病的实际负担。

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