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首页> 外文期刊>Journal of Clinical Microbiology >Pulsed-Field Gel Electrophoresis (PFGE) Analysis of Temporally Matched Listeria monocytogenes Isolates from Human Clinical Cases, Foods, Ruminant Farms, and Urban and Natural Environments Reveals Source-Associated as Well as Widely Distributed PFGE Types
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Pulsed-Field Gel Electrophoresis (PFGE) Analysis of Temporally Matched Listeria monocytogenes Isolates from Human Clinical Cases, Foods, Ruminant Farms, and Urban and Natural Environments Reveals Source-Associated as Well as Widely Distributed PFGE Types

机译：从人的临床病例，食品，反刍农场以及城市和自然环境中临时匹配的单核细胞增生李斯特菌分离株的脉冲场凝胶电泳（PFGE）分析揭示了来源相关以及分布广泛的PFGE类型

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A total of 495 temporally and geographically matched Listeria monocytogenes isolates from human clinical cases, foods, ruminant farms, and urban and natural environments were used to investigate L. monocytogenes pulsed-field gel electrophoresis (PFGE) type diversity. Two-enzyme (AscI and ApaI) PFGE discriminated 310 PFGE types and exhibited higher overall discriminatory power (Simpson's index of discrimination [D] = 0.995) than either EcoRI ribotyping (D = 0.950) or AscI or ApaI single-enzyme PFGE (D = 0.992 for both). Seven PFGE types showed significant associations with specific sources, including one and four PFGE types, respectively, associated with human clinical cases and foods. Spatial analysis of 13 PFGE types occurring >5 times showed that two PFGE types were specific to a single processing facility each, where they appear to have persisted over time. Nine PFGE types were geographically widespread and occurred among isolates from multiple sources. For example, a PFGE type that matched isolates from listeriosis outbreaks in Los Angeles and Switzerland occurred among isolates from farms (n = 7), human clinical cases (n = 4), environmental sources (n = 3), and foods (n = 1). Our data indicate that (i) PFGE is highly discriminatory for the subtyping of L. monocytogenes, (ii) some L. monocytogenes PFGE types are associated with specific sources, and (iii) some L. monocytogenes PFGE types are widely distributed and appear to be stable and pandemic. Large PFGE type databases representing isolates from different sources are thus needed to appropriately interpret subtype data in epidemiological investigations and to identify common as well as source-specific PFGE types.
机译：从人的临床病例，食品，反刍动物农场以及城市和自然环境中提取了总共495个在时间和地理上匹配的单核细胞增生李斯特菌菌株，以调查 L。单核细胞增生脉冲场凝胶电泳（PFGE）类型多样性。两种酶（AscI和ApaI）PFGE可以识别310种PFGE类型，并且比任何一种EcoRI核糖分型法（ D = 0.950）或AscI或ApaI单酶PFGE（两者的 D = 0.992）。七种PFGE类型与特定来源显着相关，包括分别与人类临床病例和食品相关的一种和四种PFGE类型。对发生次数超过5次的13种PFGE类型进行的空间分析表明，两种PFGE类型特定于单个处理设备，它们似乎随着时间的推移而持续存在。九种PFGE类型在地理上分布广泛，并发生在来自多种来源的分离株中。例如，在来自农场（ n = 7）和人类临床病例（ n = 4）的分离株中，出现了与洛杉矶和瑞士的李斯特菌病暴发的分离株相匹配的PFGE类型。 ，环境来源（ n = 3）和食物（ n = 1）。我们的数据表明（i）PFGE对于 L的亚型具有很高的歧视性。单核细胞增生，（ii）一些 L。单核细胞增生病的PFGE类型与特定来源有关，（iii）一些 L。单核细胞增生病 PFGE类型分布广泛，似乎稳定且流行。因此，需要大型PFGE类型数据库来代表来自不同来源的分离株，以便在流行病学调查中适当地解释亚型数据，并识别常见的和特定于源头的PFGE类型。

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