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首页> 外文期刊>Journal of Clinical Microbiology >Isolation and Characterization of Bartonella bacilliformis from an Expatriate Ecuadorian
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Isolation and Characterization of Bartonella bacilliformis from an Expatriate Ecuadorian

机译：来自外籍厄瓜多尔人的细菌巴尔通体的分离和鉴定

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Carrion's disease is typically biphasic with acute febrile illness characterized by bacteremia and severe hemolytic anemia (Oroya fever), followed by benign, chronic cutaneous lesions (verruga peruana). The causative agent, Bartonella bacilliformis, is endemic in specific regions of Peru and Ecuador. We describe atypical infection in an expatriate patient who presented with acute splenomegaly and anemia 3 years after visiting Ecuador. Initial serology and PCR of the patient's blood and serum were negative for Bartonella henselae, Bartonella quintana, and B. bacilliformis. Histology of splenic biopsy was suggestive of bacillary angiomatosis, but immunohistochemistry ruled out B. henselae and B. quintana. Bacilli (isolate EC-01) were subsequently cultured from the patient's blood and analyzed using multilocus sequence typing, protein gel electrophoresis with Western blotting, and an immunofluorescence assay (IFA) against a panel of sera from patients with Oroya fever in Peru. The EC-01 nucleotide sequences (gltA and internal transcribed spacer) and protein band banding pattern were most similar to a subset of B. bacilliformis isolates from the region of Caraz, Ancash, in Peru, where B. bacilliformis is endemic. By IFA, the patient's serum reacted strongly to two out of the three Peruvian B. bacilliformis isolates tested, and EC-01 antigen reacted with 13/20 Oroya fever sera. Bacilliary angiomatosis-like lesions were also detected in the spleen of the patient, who was inapparently infected with B. bacilliformis and who presumably acquired infection in a region of Ecuador where B. bacilliformis was not thought to be endemic. This study suggests that the range of B. bacilliformis may be expanding from areas of endemicity in Ecuador and that infection may present as atypical clinical disease.
机译：腐肉病通常是两相的，以细菌性和严重的溶血性贫血（Oroya热）为特征的急性发热性疾病，然后是良性，慢性皮肤病变（verruga peruana）。致病菌 Bartonella bacilliformis ，在秘鲁和厄瓜多尔的特定地区流行。我们描述了一名外来患者的非典型感染，该患者在访问厄瓜多尔3年后出现急性脾肿大和贫血。病人的血液和血清的初始血清学和PCR均对亨氏巴尔通体，巴尔通体昆塔纳和 B阴性。芽孢杆菌。脾活检的组织学提示细菌性血管瘤病，但免疫组织化学排除了 B。 henselae 和 B。金塔纳州。随后从患者血液中培养芽孢杆菌（分离出的EC-01），并使用多基因座序列分型，蛋白质凝胶电泳和Western印迹以及针对秘鲁Oroya热患者血清的免疫荧光测定（IFA）进行分析。 EC-01核苷酸序列（ gltA 和内部转录的间隔区）和蛋白条带模式与 B的一个子集最为相似。芽孢杆菌来自秘鲁安卡什（Ancash）的Caraz地区， B。芽孢杆菌是地方病。通过IFA，患者的血清对三个秘鲁 B中的两个有强烈反应。测试了芽孢杆菌分离株，并且EC-01抗原与13/20 Oroya发烧血清反应。在患者的脾脏中也检测到了细菌性血管瘤样病变，他们显然没有感染 B。细菌，大概是在厄瓜多尔的一个 B地区感染的。芽孢杆菌被认为不是地方病。这项研究表明 B的范围。杆状菌可能从厄瓜多尔的流行地区扩展，这种感染可能表现为非典型的临床疾病。

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