Rapid Group-, Serotype-, and Vaccine Strain-Specific Identification of Poliovirus Isolates by Real-Time Reverse Transcription-PCR Using Degenerate Primers and Probes Containing Deoxyinosine Residues

David R. Kilpatrick; Chen-Fu Yang; Karen Ching; Annelet Vincent; Jane Iber; Ray Campagnoli; Mark Mandelbaum; Lina De; Su-Ju Yang; Allan Nix; Olen M. Kew

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Rapid Group-, Serotype-, and Vaccine Strain-Specific Identification of Poliovirus Isolates by Real-Time Reverse Transcription-PCR Using Degenerate Primers and Probes Containing Deoxyinosine Residues

机译：使用简并引物和含脱氧肌苷残基的探针通过实时逆转录PCR快速鉴定脊髓灰质炎病毒分离物的组，血清型和疫苗株特异性。

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We have adapted our previously described poliovirus diagnostic reverse transcription-PCR (RT-PCR) assays to a real-time RT-PCR (rRT-PCR) format. Our highly specific assays and rRT-PCR reagents are designed for use in the WHO Global Polio Laboratory Network for rapid and large-scale identification of poliovirus field isolates.

机译：我们已经将我们先前描述的脊髓灰质炎病毒诊断性逆转录PCR（RT-PCR）分析调整为实时RT-PCR（rRT-PCR）格式。我们的高度特异性测定法和rRT-PCR试剂旨在用于WHO全球脊髓灰质炎实验室网络，以快速，大规模地鉴定脊髓灰质炎病毒现场分离株。

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《Journal of Clinical Microbiology》 |2009年第6期|共3页
作者
David R. Kilpatrick; Chen-Fu Yang; Karen Ching; Annelet Vincent; Jane Iber; Ray Campagnoli; Mark Mandelbaum; Lina De; Su-Ju Yang; Allan Nix; Olen M. Kew;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Genogroup I and II Noroviruses Detected in Stool Samples by Real-Time Reverse Transcription-PCR Using Highly Degenerate Universal Primers [J] . Gary P. Richards, Michael A. Watson, Rebecca L. Fankhauser, Applied and Environmental Microbiology . 2004,第12期

机译：使用高度简并的通用引物通过实时逆转录PCR PCR检测粪便样品中的基因组I和II诺如病毒
2. Genogroup I and II Noroviruses Detected in Stool Samples by Real-Time Reverse Transcription-PCR Using Highly Degenerate Universal Primers [J] . Gary P. Richards, Michael A. Watson, Rebecca L. Fankhauser, Applied and Environmental Microbiology . 2004,第12期

机译：使用高度简并的通用引物通过实时逆转录PCR PCR检测粪便样品中的基因组I和II诺如病毒
3. Broad Detection of Diverse H5 and H7 Hemagglutinin Genes of Avian Influenza Viruses by Real-Time Reverse Transcription-PCR Using Primer and Probe Sets Containing Mixed Bases [J] . Kenji Tsukamoto, Daigo Noguchi, Koutaro Suzuki, Journal of Clinical Microbiology . 2010,第11期

机译：实时逆转录PCR使用引物和含混合碱基的探针组对禽流感病毒的不同H5和H7血凝素基因进行广泛检测
4. Development of Real-Time PCR Primer and Probe Sets for Detecting Degenerated and Non-degenerated Forms of the Butanol-Producing Bacterium Clostridium acetobutylicum ATCC 824 [C] . Sun-Mi Lee, Min Ok Cho, Youngsoon Um, Symposium on biotechnology for fuels and chemicals . 2010

机译：用于检测丁醇产物脱苯乙烯酸梭菌ATCC 824的丁醇产物菌梭菌梭菌的退化和非退化形式的实时PCR引物和探针组
5. Rapid Group- Serotype- and Vaccine Strain-Specific Identification of Poliovirus Isolates by Real-Time Reverse Transcription-PCR Using Degenerate Primers and Probes Containing Deoxyinosine Residues [O] . David R. Kilpatrick, Chen-Fu Yang, Karen Ching, 2009

机译：使用简并引物和含脱氧肌苷残基的探针通过实时逆转录PCR快速鉴定脊髓灰质炎病毒分离物的组血清型和疫苗株特异性。
6. Rapid Group-, Serotype-, and Vaccine Strain-Specific Identification of Poliovirus Isolates by Real-Time Reverse Transcription-PCR Using Degenerate Primers and Probes Containing Deoxyinosine Residues▿ ‡ [O] . Kilpatrick, David R., Yang, Chen-Fu, Ching, Karen, 2009

机译：使用简并引物和含脱氧肌苷残基的探针通过实时逆转录PCR快速鉴定脊髓灰质炎病毒分离物的组，血清型和疫苗株特异性

Rapid Group-, Serotype-, and Vaccine Strain-Specific Identification of Poliovirus Isolates by Real-Time Reverse Transcription-PCR Using Degenerate Primers and Probes Containing Deoxyinosine Residues

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