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首页> 外文期刊>Journal of Clinical Microbiology >Association of Unique, Isolated Treponemes with Bovine Digital Dermatitis Lesions
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Association of Unique, Isolated Treponemes with Bovine Digital Dermatitis Lesions

机译：独特的孤立性色氨酸与牛数字皮肤炎病变的关联

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This study used a PCR-based approach targeting 16S rRNA gene fragments to determine the occurrence and association of the three bovine digital dermatitis (BDD) treponeme phylogroups within lesions found in cattle from the United Kingdom. Examination of 51 BDD lesions collected from infected cattle across the United Kingdom revealed that BDD treponeme group 1 (Treponema medium/Treponema vincentii-like), group 2 (Treponema phagedenis-like), and group 3 (Treponema putidum/Treponema denticola-like) were present in 96.1%, 98%, and 76.5% of BDD lesions, respectively. The three phylogroups were present together in 74.5% of lesions. The PCR assays enabled the isolation of further treponeme strains from previously mixed primary BDD lesion cultures. Here a representative from each of the three distinct treponeme phylogroups was isolated from a single BDD lesion for the first time. These data highlight the extent to which this disease is polytreponemal. Immunohistochemistry and electron microscopy were used to investigate lesional hoof tissues, resulting in treponemes being identified copiously in hair follicles and sebaceous glands, suggesting a potential route of exit and/or entry for these pathogens. This study gives further evidence for the importance of the three treponeme groups in BDD pathogenesis and reiterates the value of molecular genetic approaches for isolating and identifying fastidious anaerobes.
机译：这项研究使用基于PCR的方法靶向16S rRNA基因片段，以确定在英国的牛中发现的病变中三个牛数字性皮炎（BDD）色氨酸系统群的发生和关联。对从英国感染牛身上采集的51种BDD病变进行的检查显示，BDD截短蛋白组1（ Treponema medium / Treponema vincentii -like），第二组（ Treponema phagedenis 样）和第3组（ Treponema putidum / Treponema denticola 样）分别占BDD病变的96.1％，98％和76.5％ ， 分别。这三个系统族共存在于74.5％的病变中。 PCR测定法能够从先前混合的原发性BDD病变培养物中分离出进一步的treponeme菌株。在这里，首次从一个单一的BDD病变中分离出三个不同的treponeme系统群中的每个代表。这些数据突显了这种疾病的程度。免疫组织化学和电子显微镜用于研究病变蹄组织，导致在毛囊和皮脂腺中大量鉴定了色氨酸，暗示了这些病原体可能的出入途径。这项研究提供了进一步的证据，证明这三个分子群在BDD发病机理中的重要性，并重申了分子遗传学方法在分离和鉴定优良厌氧菌中的价值。

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