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首页> 外文期刊>Veterinary Microbiology >A molecular epidemiology of treponemes in beef cattle digital dermatitis lesions and comparative analyses with sheep contagious ovine digital dermatitis and dairy cattle digital dermatitis lesions
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A molecular epidemiology of treponemes in beef cattle digital dermatitis lesions and comparative analyses with sheep contagious ovine digital dermatitis and dairy cattle digital dermatitis lesions

机译：牛牛数字性皮炎病变中色氨酸的分子流行病学研究及与绵羊传染性绵羊数字性皮炎和奶牛数字性皮炎病害的比较分析

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Bovine digital dermatitis (BDD) is an infective foot disease commonly reported in dairy cattle where Treponema are considered as the primary causative infectious agents. There still remains little definitive information on the etiology of BDD in beef cattle suggesting further investigations are warranted. Beef BDD lesions (n = 34) and healthy beef foot tissues (n = 38) were analysed by PCR for three BDD-associated Treponema phylogroups and also for Dichelobacter nodosus and Fusobacterium necrophorum. Spirochete culture was attempted on all BDD lesion samples. One or more BDD-associated Treponema phylogroups were detected in 100% of beef BDD lesions. "Treponema medium/Treponema vincentii-like", "Treponema phagedenis-like" and Treponema pedis spirochetes were identified in 27/34 (79%), 31/34 (91%) and 24/34 (71%) of BDD lesions, respectively. No BDD-associated treponeme DNA was amplified from beef healthy foot tissues. D. nodosus and F. necrophorum were present in 24/34 (71%) and 15/34 (44%) of lesions and 10/38 (26%) and 12/38 (32%) of healthy foot tissues, respectively. Twenty spirochetes were isolated from beef BDD lesions; 19 were representatives of the three BDD-associated Treponema phylogroups. One spirochete isolate shared less than 97% 16S rRNA gene similarity to the three cultivable BDD-associated Treponema phylogroups and therefore may represent a novel taxa of Treponema. Upon comparison, sheep contagious ovine digital dermatitis (CODD), dairy cattle and beef cattle BDD lesions appear to have extremely similar bacteriological data and therefore provides evidence of a shared etiopathogenesis posing concerns for cross-species transmission. (C) 2015 Elsevier B.V. All rights reserved.
机译：牛数字皮肤炎（BDD）是一种常见的感染性足部疾病，在奶牛中，梅毒螺旋体被认为是主要的病原性传染源。关于肉牛中BDD病因的确切信息仍然很少，这表明需要进行进一步的研究。通过PCR分析了牛肉BDD病变（n = 34）和健康的牛脚组织（n = 38）的三个与BDD相关的梅毒螺旋体系统群，以及结节双歧杆菌和坏死镰刀菌。已尝试在所有BDD病变样品上进行螺旋藻培养。在100％的牛肉BDD病变中检测到一个或多个与BDD相关的梅毒螺旋体系统群。在BDD病变的27/34（79％），31/34（91％）和24/34（71％）的BDD病变中发现了“中型/梅毒螺旋体”，“食性密螺旋体”和足癣螺旋体。分别。没有从牛肉健康的足部组织中扩增出与BDD相关的截短蛋白DNA。 D. nodosus和坏死镰刀菌分别存在于健康足部组织的24/34（71％）和15/34（44％）和10/38（26％）和12/38（32％）的病变中。从牛肉BDD病变中分离出二十个螺旋体; 19位代表了三个与BDD相关的螺旋体系统群。一个螺旋体分离物与三个可培养的BDD相关的梅毒螺旋体系统群共享少于97％的16S rRNA基因相似性，因此可能代表了新的梅毒螺旋体类群。比较起来，绵羊传染性绵羊数字皮肤炎（CODD），奶牛和肉牛BDD病变似乎具有极其相似的细菌学数据，因此提供了共同病因的证据，引起跨物种传播。 （C）2015 Elsevier B.V.保留所有权利。

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