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首页> 外文期刊>Journal of Clinical Microbiology >Phylogenetic Analysis of a Virulent Borrelia Species Isolated from Patients with Relapsing Fever
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Phylogenetic Analysis of a Virulent Borrelia Species Isolated from Patients with Relapsing Fever

机译：从复发性发热患者中分离出强毒疏螺旋体的系统发育分析

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Multilocus sequence analysis (MLSA) was used to clarify the taxonomic status of a virulent Borrelia organism previously isolated from patients with relapsing fever and from ticks in Spain that is designated the Spanish relapsing fever (SRF) Borrelia. This species has been used extensively in experimental infection models because of its continued virulence. Seven genes were amplified to analyze the phylogenetic relationships among several Spanish isolates of SRF Borrelia and other relapsing fever Borrelia species. The genes targeted in this study included rrs and flaB, which have commonly been used in phylogenetic studies; the rrf-rrl intergenic spacer (IGS), which is highly discriminatory; and four additional genes, p66, groEL, glpQ, and recC, which are located on the chromosome and which have therefore evolved in a clonal way. The species included in this study were Borrelia duttonii, B. recurrentis, B. crocidurae, and B. hispanica as Old World Borrelia species and B. turicatae and B. hermsii as New World Borrelia species. The results obtained by MLSA of the SRF Borrelia on the basis of 1% of the genomic sequence data analyzed confirmed that the SRF Borrelia isolates are B. hispanica. However, the prototype isolates of B. hispanica used in this study have an uncertain history and display unique phenotypic characteristics that are not shared with the SRF Borrelia. Therefore, we propose to use strain SP1, isolated from a relapsing fever patient in 1994 in southern Spain, as the type strain for B. hispanica.
机译：使用多基因座序列分析（MLSA）来阐明以前从西班牙的复发性发热患者和tick中分离出的强毒 Borrelia 生物的生物分类状况，该微生物被称为西班牙复发性发热（SRF）疏螺旋体。由于其持续的毒力，该物种已广泛用于实验感染模型。扩增了七个基因，以分析西班牙分离的SRF Borrelia 和其他复发性发烧 Borrelia 物种的种系关系。这项研究的目标基因包括 rrs 和 flaB ，它们通常用于系统发育研究。 rrf-rrl 基因间隔子（IGS），具有很高的歧视性;还有另外四个基因 p66 ， groEL ， glpQ 和 recC ，它们位于染色体上，因此已经以克隆方式进化。这项研究包括的物种是 Borrelia duttonii ， B。复发， B。 crocidurae 和 B。 hispanica 作为旧世界的 Borrelia 和 B。 turicatae 和 B。 hermsii 作为新世界 Borrelia 物种。 MLSA根据SRF Borrelia 的1％基因组序列数据获得的结果证实，SRF Borrelia 分离株为 B。西班牙裔。但是， B的原型分离株。本研究中使用的hispanica 具有不确定的历史，并表现出独特的表型特征，而SRF Borrelia 则没有。因此，我们建议使用1994年从西班牙南部一名复发性发热患者中分离出的SP1株作为B型株。西班牙裔。

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