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首页> 外文期刊>Journal of Clinical Microbiology >Rapid Detection of aac(6′)-Ib-cr Quinolone Resistance Gene by Pyrosequencing
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Rapid Detection of aac(6′)-Ib-cr Quinolone Resistance Gene by Pyrosequencing

机译:焦磷酸测序快速检测aac(6')-Ib-cr喹诺酮耐药基因

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Pyrosequencing was used to rapidly detect aac(6)-Ib and aac(6)-Ib-cr genes. This plasmid-mediated quinolone resistance determinant is increasing in extended-spectrum beta-lactamase-producing Enterobacteriaceae. This method is faster and more cost-effective than the methods previously described. Sequences obtained with this pyrosequencing method showed 100% concordance with conventional sequencing.
机译:焦磷酸测序用于快速检测 aac(6 ')-Ib aac(6 ')-Ib-cr 基因。该质粒介导的喹诺酮抗性决定簇在产生广谱β-内酰胺酶的肠杆菌科中正在增加。该方法比先前描述的方法更快且更具成本效益。用这种焦磷酸测序方法获得的序列与常规测序显示出100%的一致性。

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