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首页> 外文期刊>Journal of Clinical Microbiology >Development of a Rapid ATP Bioluminescence Assay for Biocidal Susceptibility Testing of Rapidly Growing Mycobacteria
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Development of a Rapid ATP Bioluminescence Assay for Biocidal Susceptibility Testing of Rapidly Growing Mycobacteria

机译:快速ATP分光光度法快速增长的分枝杆菌的药敏试验的发展。

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An ATP-based biocide susceptibility assay for mycobacteria was developed by optimizing the cell lysis and assay conditions. Compared to the conventional agar plating method, the assay was rapid (1.5 h) and showed high sensitivity and specificity as determined by receiver operating characteristic (ROC) analysis. The test species, Mycobacterium immunogenum, M. chelonae, and M. abscessus, showed various susceptibilities to the glutaraldehyde- and isothiazolone-based test biocides.
机译:通过优化细胞裂解和分析条件,开发了基于ATP的分枝杆菌药敏试验。与传统的琼脂平板接种方法相比,该测定速度快(1.5小时),并显示出高灵敏度和特异性,这是通过接受者操作特征(ROC)分析确定的。测试种 M 分枝杆菌 免疫原 M。 chelonae M。脓肿对基于戊二醛和异噻唑酮的测试杀菌剂表现出不同的敏感性。

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