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NOVEL METHODS FOR RAPID DRUG SUSCEPTIBILITY TESTING AND DRUG SCREENS FOR GROWING AND NON-GROWING CELLS OF PROKARYOTIC AND EUKARYOTIC ORIGIN
NOVEL METHODS FOR RAPID DRUG SUSCEPTIBILITY TESTING AND DRUG SCREENS FOR GROWING AND NON-GROWING CELLS OF PROKARYOTIC AND EUKARYOTIC ORIGIN
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机译：用于原核和真核细胞生长和非生长细胞的快速药物敏感性测试和药物筛查的新方法
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摘要
The presently disclosed subject matter provides methods, compositions, and kits for extremely rapid assessment of drug susceptibility of growing as well as non-growing cells of prokaryotic and also eukaryotic origins, without the need for organism growth, which is in contrast to the current growth-dependent methods.This growth-independent new methodology achieves extremely rapid drug susceptibility testing results previously not possible. Methods for rapidly identifying compounds with inhibitory activity against both growing and non-growing cells in real-time continuous manner with previously unachievable efficiency in drug screens using the novel methodology are also provided. Cell cultures are incubated with a drug or test agent and with a staining mixture containing a first fluorescent dye indicative the emission of which is indicative of live cells (e.g. SYBR Green I) and a second fluorescent dye indicative emitting a different color, the emission of which is indicative of dead cells (e.g. Propidium iodide). The ratio of the intensities of the emitted fluorescence of the dyes is indicative of the percentage live and dead cells in the culture.
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机译：当前公开的主题提供了用于非常快速地评估原核以及真核来源的生长以及非生长细胞的药物敏感性的方法，组合物和试剂盒，而无需生物生长，这与当前的生长相反依赖于方法的方法。这种与生长无关的新方法可以实现以前不可能的极其快速的药物敏感性测试结果。还提供了使用新颖的方法以实时连续的方式快速鉴定具有抑制活性的化合物的方法，所述抑制活性以实时连续的方式在药物筛选中具有以前无法实现的效率。细胞培养物与药物或测试剂一起孵育，并与含有第一种荧光染料的染色混合物一起孵育，该荧光染料指示放射的是活细胞（例如SYBR Green I），第二种荧光染料指示放射的是不同的颜色，指示死细胞（例如碘化丙啶）。染料发出的荧光强度的比值指示培养物中活细胞和死细胞的百分比。
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