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首页> 外文期刊>Journal of Clinical Microbiology >Molecular and Antimicrobial Susceptibility Analyses Distinguish Clinical from Bovine Escherichia coli O157 Strains
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Molecular and Antimicrobial Susceptibility Analyses Distinguish Clinical from Bovine Escherichia coli O157 Strains

机译：分子和抗菌药性分析与牛大肠杆菌O157菌株的临床区别

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A population-based study combining (i) antimicrobial, (ii) genetic, and (iii) virulence analyses with molecular evolutionary analyses revealed segregative characteristics distinguishing human clinical and bovine Escherichia coli O157 strains from western Canada. Human (n = 50) and bovine (n = 50) strains of E. coli O157 were collected from Saskatchewan and Manitoba in 2006 and were analyzed by using the six-marker lineage-specific polymorphism assay (LSPA6), antimicrobial susceptibility analysis, the colicin assay, plasmid and virulence profiling including the eae, ehxA, espA, iha, stx1, stx2, stx2c, stx2d, stx2d-activatable, stx2e, and stx2f virulence-associated genes, and structure analyses. Multivariate logistic regression and Fisher's exact test strongly suggested that antimicrobial susceptibility was the most distinctive characteristic (P = 0.00487) associated with human strains. Among all genetic, virulence, and antimicrobial determinants, resistance to tetracycline (P < 0.000) and to sulfisoxazole (P < 0.009) were the most strongly associated segregative characteristics of bovine E. coli O157 strains. Among 11 virulence-associated genes, stx2c showed the strongest association with E. coli O157 strains of bovine origin. LSPA6 genotyping showed the dominance of the lineage I genotype among clinical (90%) and bovine (70%) strains, indicating the importance of lineage I in O157 epidemiology and ecology. Population structure analysis revealed that the more-diverse bovine strains came from a unique group of strains characterized by a high degree of antimicrobial resistance and high frequencies of lineage II genotypes and stx2c variants. These findings imply that antimicrobial resistance generated among bovine strains of E. coli O157 has a large impact on the population of this human pathogen.
机译：一项基于人群的研究将（i）抗菌，（ii）遗传和（iii）毒力分析与分子进化分析相结合，揭示了将人临床和牛大肠杆菌O157菌株与加拿大西部区分开的分离特征。 2006年从萨斯喀彻温省和曼尼托巴省收集了人（ n = 50）和牛（ n = 50）大肠杆菌O157菌株，并使用六标记进行了分析谱系特异性多态性分析（LSPA6），抗菌药敏感性分析，大肠菌素分析，质粒和毒力分析，包括 ea ， ehxA ， espA ， iha ， stx 1 ， stx 2 ， stx 2c ， stx 2d ， stx 2d可激活， stx 2e 和 stx 2f 毒力相关基因，并进行结构分析。多元逻辑回归和Fisher精确检验强烈表明，抗菌素敏感性是与人类菌株相关的最独特特征（ P = 0.00487）。在所有遗传，毒力和抗菌素决定因素中，对牛四环素（ P <0.000）和对磺胺异恶唑（ P <0.009）的抵抗力与牛E的分离力最相关大肠杆菌O157菌株。在11个与毒力相关的基因中， stx 2c 显示与牛源大肠杆菌O157菌株的关联最强。 LSPA6基因分型显示临床（90％）和牛（70％）菌株中I谱系基因型的优势，表明I谱系在O157流行病学和生态学中的重要性。种群结构分析表明，多样性更高的牛菌株来自一组独特的菌株，这些菌株具有高度的抗药性和高频率的谱系II基因型和 stx 2c 变体。这些发现表明，在大肠杆菌O157的牛株之间产生的抗药性对该人类病原体的种群有很大的影响。

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