Development and Optimization of a Real-Time PCR Assay for Detection of Herpes Simplex and Varicella-Zoster Viruses in Skin and Mucosal Lesions by Use of the BD Max Open System

Ana María Cárdenas; Paul H. Edelstein; Kevin Alby

首页> 外文期刊>Journal of Clinical Microbiology >Development and Optimization of a Real-Time PCR Assay for Detection of Herpes Simplex and Varicella-Zoster Viruses in Skin and Mucosal Lesions by Use of the BD Max Open System

【24h】

Development and Optimization of a Real-Time PCR Assay for Detection of Herpes Simplex and Varicella-Zoster Viruses in Skin and Mucosal Lesions by Use of the BD Max Open System

机译：通过使用BD Max开放系统开发和优化实时荧光定量PCR检测皮肤和粘膜病变中的单纯疱疹和水痘带状疱疹病毒

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

We transitioned laboratory-developed PCR assays for herpes simplex virus 1 (HSV-1), HSV-2, and varicella-zoster virus (VZV) to the BD Max system by using BD Max open system reagents. After optimization, the agreement with the reference PCR assay was 100% (123/123) for HSV-1, 96.7% (119/123) for HSV-2, and 100% (60/60) for VZV using retrospective clinical samples.

机译：通过使用BD Max开放系统试剂，我们将实验室开发的单纯疱疹病毒1（HSV-1），HSV-2和水痘带状疱疹病毒（VZV）的PCR测定法过渡到BD Max系统。优化后，使用回顾性临床样本，与参考PCR分析的一致性为HSV-1为100％（123/123），HSV-2为96.7％（119/123）和VZV为100％（60/60）。

著录项

来源
《Journal of Clinical Microbiology》 |2014年第12期|共2页
作者
Ana María Cárdenas; Paul H. Edelstein; Kevin Alby;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种
中图分类医学微生物学（病原细菌学、病原微生物学）;
关键词

相似文献

外文文献
中文文献
专利

1. Development and Validation of a Laboratory-Developed Multiplex Real-Time PCR Assay on the BD Max System for Detection of Herpes Simplex Virus and Varicella-Zoster Virus DNA in Various Clinical Specimens [J] . Sylvie Pillet, Paul O. Verhoeven, Amélie Epercieux, Journal of Clinical Microbiology . 2015,第6期

机译：用于检测各种临床标本中的单纯疱疹病毒和水痘-带状疱疹病毒DNA的BD Max系统的实验室实时多重PCR检测的开发和验证
2. Detection of herpes simplex virus type 1, herpes simplex virus type 2 and varicella-zoster virus in skin lesions. Comparison of real-time PCR, nested PCR and virus isolation. [J] . Schmutzhard J, Merete Riedel H, Zweygberg Wirgart B, Journal of clinical virology: The official publication of the Pan American Society for Clinical Virology . 2004,第2期

机译：检测皮肤病变中的1型单纯疱疹病毒和2型单纯疱疹病毒和水痘带状疱疹病毒。实时PCR，巢式PCR和病毒分离的比较。
3. Development of a multiplex real-time PCR for the simultaneous detection of herpes simplex and varicella zoster viruses in cerebrospinal fluid and lesion swab specimens [J] . Wong Anita A., Pabbaraju Kanti, Wong Sallene, Journal of Virological Methods . 2016,第Null期

机译：开发用于同时检测脑脊液和病变拭子标本中单纯疱疹和水痘带状疱疹病毒的多重实时PCR
4. RAPID DETECTION OF SWINE INFLUENZA VIRUSES USING A REAL-TIME PCR ASSAY [C] . JA Richt, DF Clouser, KM Lager, Congress of the International Pig Veterinary Society . 2002

机译：使用实时PCR测定快速检测猪流感病毒
5. Development and Application of a Simple Molecular Short RT-PCR Assay for Detection and Differentiation of Influenza A Viruses. [D] . Benrahoma, Ghada Mohamed. 2013

机译：一种简单的分子短RT-PCR检测和鉴别甲型流感病毒的方法的开发和应用。
6. Development and Optimization of a Real-Time PCR Assay for Detection of Herpes Simplex and Varicella-Zoster Viruses in Skin and Mucosal Lesions by Use of the BD Max Open System [O] . Ana María Cárdenas, Paul H. Edelstein, Kevin Alby 2014

机译：通过使用BD Max开放系统开发和优化实时荧光定量PCR检测皮肤和粘膜病变中的单纯疱疹和水痘带状疱疹病毒
7. Multiplex PCR for Detection of Herpes Simplex Viruses Type-1 and Type-2, Cytomegalovirus, Varicella-zoster Virus, and Adenovirus in Ocular Viral Infections [O] . Ahmed Nishat H, Gita Satpathy, Rohan Chawla, 2021

机译：用于检测单纯疱疹病毒类型-1和2型，塞细胞病毒，种植体 - 带状疱疹病毒的多重PCR和眼病毒感染中的腺病毒

Development and Optimization of a Real-Time PCR Assay for Detection of Herpes Simplex and Varicella-Zoster Viruses in Skin and Mucosal Lesions by Use of the BD Max Open System

摘要

著录项

相似文献

相关主题

期刊订阅