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首页> 外文期刊>Journal of Clinical Microbiology >Ability of the Digene Hybrid Capture II Test To Identify Chlamydia trachomatis and Neisseria gonorrhoeae in Cervical Specimens
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Ability of the Digene Hybrid Capture II Test To Identify Chlamydia trachomatis and Neisseria gonorrhoeae in Cervical Specimens

机译：Digene Hybrid Capture II测试在宫颈标本中鉴定沙眼衣原体和淋病奈瑟菌的能力

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The Digene Hybrid Capture II (HCII CT/GC) test is a combination test designed to detect Chlamydia trachomatis andNeisseria gonorrhoeae in a single specimen. It is a nucleic acid hybridization test which uses signal amplification to increase sensitivity. We compared its performance to that of culture on cervical specimens from 1,370 women. Direct fluorescent-antibody assay was used to resolve discrepant results for C. trachomatis. Samples were collected with a proprietary cervical brush or with endocervical swabs. The HCII CT/GC test proved to be sensitive and specific in detecting these organisms. Compared to N. gonorrhoeaeculture, it had a sensitivity of 93% (87/94) and a specificity of 98.5% (1,244/1,263). Compared to C. trachomatis culture, the sensitivity was 97.7% (129/132) and specificity was 98.2% (1,216/1,238). Testing of some specimens with discrepant results by PCR suggested that the test would actually prove to be even more specific if it were compared to a nucleic acid amplification test (NAAT). The sensitivity of C. trachomatis culture was somewhat less, at 88.6% (117/132). The endocervical brush appeared to be better than Dacron swabs for collecting specimens. The HCII CT/GC test offers an attractive format that allows simultaneous detection of C. trachomatis and N. gonorrhoeae with a single specimen. An initial positive result is followed by repeat tests with probes to identify chlamydiae or gonococci. This test is more sensitive than C. trachomatis culture and is at least as sensitive as culture for gonococci. It deserves further evaluation and comparison with NAATs and may well offer an attractive alternative for diagnosis and screening of these infections.
机译：Digene Hybrid Capture II（HCII CT / GC）测试是一项组合测试，旨在检测单个样本中的沙眼衣原体和淋病奈瑟氏菌。这是一种核酸杂交测试，它使用信号放大来提高灵敏度。我们将其性能与在1,370名女性的宫颈标本上的培养性能进行了比较。直接荧光抗体测定法用于解析C的差异结果。沙眼。用专用宫颈刷或宫颈拭子收集样品。 HCII CT / GC测试证明对检测这些生物体具有敏感性和特异性。与 N相比。淋球菌培养，其敏感性为93％（87/94），特异性为98.5％（1,244 / 1,263）。与 C相比。沙眼培养，敏感性为97.7％（129/132），特异性为98.2％（1,216 / 1,238）。通过PCR对某些标本结果不一致的测试表明，如果将其与核酸扩增测试（NAAT）进行比较，则该测试实际上会更加特异性。 C的灵敏度。沙眼培养较少，为88.6％（117/132）。子宫颈刷似乎比涤纶棉签更好地收集了标本。 HCII CT / GC测试提供了一种有吸引力的格式，可以同时检测 C。沙眼和 N。淋病菌只有一个标本。最初的阳性结果后，用探针进行重复测试以鉴定衣原体或淋球菌。此测试比 C更为敏感。沙眼培养，至少与淋球菌培养一样敏感。它值得进一步评估和与NAAT进行比较，并可能为诊断和筛查这些感染提供有吸引力的替代方法。

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