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首页> 外文期刊>Journal of Clinical Microbiology >Biochemical Differentiation and Comparison of Desulfovibrio Species and Other Phenotypically Similar Genera
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Biochemical Differentiation and Comparison of Desulfovibrio Species and Other Phenotypically Similar Genera

机译：脱硫弧菌和其他表型相似属的生化分化和比较

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Seventeen human clinical isolates representing four species of Desulfovibrio were characterized using 16S rRNA gene sequences and tests for catalase, indole, nitrate, bile, urease, formate-fumarate stimulation, desulfoviridin, motility, and hydrogen sulfide production, plus susceptibility to antimicrobial agents. Eighty additional strains representing 10 phenotypically similar genera (Bilophila, Selenomonas, Capnocytophaga, Campylobacter, Bacteroides, Sutterella, Anaerobiospirillum, Dialister, Veillonella, and Mobiluncus) were included for comparison. All Desulfovibrio species produced H2S and were desulfoviridin positive, and all Desulfovibrio species except D. piger were motile. The four Desulfovibrio species could be distinguished from each other using tests for catalase, indole, nitrate, urease, and growth on bile, with the following results (positive [+], negative [?], growth [G], and no growth [NG]): for D. piger, ?,? , ?, ?, and G, respectively; for D. fairfieldensis, +, ?, +, ?, and G, respectively; for D. desulfuricans, ?,? , +, +, and NG, respectively; and for D. vulgaris, ?, +, ?,? , and G, respectively. Resistance to the 10-μg colistin disk separated the Desulfovibrio species from most of the other genera, which were usually susceptible. These simple tests were useful for characterizing the Desulfovibrio species and differentiating them from other phenotypically similar genera.
机译：使用16S rRNA基因序列表征了代表四种Desulfovibrio 的17种人类临床分离株，并对过氧化氢酶，吲哚，硝酸盐，胆汁，脲酶，甲酸-富马酸盐刺激，脱硫维西丁，运动性和硫化氢产生进行了测试，加上对抗菌剂的敏感性。代表另外10个表型相似属的80个菌株（ Bilophila ， Selenomonas ， Capnocytophaga ， Campylobacter ， Bacteroides < / em>， Sutterella ，厌氧菌属， Dialister ， Veillonella 和 Mobiluncus ）进行比较。所有 Desulfovibrio 菌种均产生H 2 S，且均为去硫磺弧菌素阳性，所有 Desulfovibrio 菌种除 D。猪人运动能力强。使用过氧化氢酶，吲哚，硝酸盐，脲酶和胆汁生长的测试可以区分四种 Desulfovibrio 物种，结果如下（阳性[+]，阴性[？]，生长[ G]，没有增长[NG]）：对于 D。猪猪，？，？ ，？，？和G分别;为 D 。 fairfieldensis ，+，？，+ 、?和G;对于 D。脱硫菌素，？，？ 、、 +，+和NG;对于 D。寻常，？，+，？，？ ，和G分别。对10μg粘菌素圆盘的抗性将 Desulfovibrio 菌种与其他大多数易感种分开。这些简单的测试对于鉴定 Desulfovibrio 菌种并将它们与其他表型相似的属区分开来很有用。

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