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首页> 外文期刊>Journal of Clinical Microbiology >Rapid Identification of Mycobacterium tuberculosis Beijing Genotypes on the Basis of the Mycobacterial Interspersed Repetitive Unit Locus 26 Signature
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Rapid Identification of Mycobacterium tuberculosis Beijing Genotypes on the Basis of the Mycobacterial Interspersed Repetitive Unit Locus 26 Signature

机译：基于分枝杆菌散布的重复单元基因座26特征快速鉴定北京结核分枝杆菌基因型

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摘要

Mycobacterium tuberculosis Beijing strains are prevalent in many parts of the world and often give rise to large institutional outbreaks. Such highly transmissible strains, often associated with multidrug resistance, are likely underrepresented in outbreaks reported from developing countries, mainly due to nonavailability of fast detection methods suitable in epidemiological surveillance studies. We evaluated a PCR assay based on amplification of mycobacterial interspersed repetitive unit locus 26 as a stand-alone method for unambiguous identification of Beijing strains. The method was used on blinded samples from 10 standard strains whose Beijing status was already confirmed by spoligotyping. All 10 strains were accurately identified, and their profiles were corroborated successfully with spoligotypes. The method was also applied to 70 different non-Beijing clinical isolates from different countries to allow discrimination of isolates. Owing to its accuracy, simplicity, and rapidity, the assay can be employed in laboratory-level testing of isolates linked to certain outbreaks. The test can also be adopted for direct application on clinical samples to save time on culturing bacilli for genotyping.
机译：结核分枝杆菌北京毒株在世界许多地方普遍存在，并经常引起大规模的机构性暴发。此类经常与多重耐药性相关的高传播菌株，在发展中国家报告的暴发中可能没有得到很好的体现，这主要是由于没有适用于流行病学监测研究的快速检测方法。我们评估了基于分枝杆菌散布的重复单位基因座26扩增的PCR分析，作为明确鉴定北京菌株的独立方法。该方法用于来自10个标准品系的盲样品，这些品系的北京地位已通过poli窃法得到证实。准确鉴定了所有10个菌株，并成功地通过Spoligotypes证实了它们的概况。该方法还应用于来自不同国家的70种不同的北京非临床分离株，以区分分离株。由于其准确性，简便性和快速性，该测定法可用于与某些暴发有关的分离株的实验室级测试。该测试还可直接用于临床样品，以节省培养细菌进行基因分型的时间。

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