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Mechanisms To Assess Gram Stain Interpretation Proficiency of Technologists at Satellite Laboratories

机译:评估卫星实验室技术人员的革兰氏染色解释能力的机制

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To address Gram stain interpretation proficiency in a satellite/centralized microbiology laboratory paradigm, two programs were devised. In quality assurance program 1, nonmicrobiology technologists at satellite laboratories were required to interpret standardized Gram-stained specimens of clinical material prepared by an experienced microbiologist at a central laboratory. In quality assurance program 2, clinical Gram stains prepared and read by the satellite laboratorians were reviewed by experienced microbiologists at the central laboratory. Satisfactory performance (94%) was achieved in quality assurance program 1. In contrast, quality assurance program 2 had a significantly lower overall performance (89%; P < 0.0001) due to poorer identification of host cells (93%) and bacteria (84%). A variety of intervention mechanisms, including continuous monitoring, resulted in overall performance improvement (P ≤ 0.006). While a technologist challenge has educational merit, having a microbiologist review previously read slides is a better indicator of the technologist's Gram stain interpretation proficiency.
机译:为了解决卫星/集中式微生物实验室范式中革兰氏染色的解释能力,设计了两个程序。在质量保证计划1中,要求卫星实验室的非微生物技术人员解释由经验丰富的微生物学家在中央实验室准备的标准化革兰氏染色临床材料标本。在质量保证计划2中,由卫星实验室工作人员准备和读取的临床革兰氏染色剂由中央实验室的资深微生物学家进行了审查。质量保证程序1达到了令人满意的性能(94%)。相反,由于对宿主细胞的识别较差,质量保证程序2的总体性能明显较低(89%; P <0.0001)。 93%)和细菌(84%)。多种干预机制,包括持续监控,导致整体绩效提高( P ≤0.006)。尽管技术人员的挑战具有教育意义,但让微生物学家进行事先阅读的幻灯片可以更好地表明技术人员的革兰氏染色解释能力。

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