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首页> 外文期刊>Journal of Clinical Microbiology >Bordetella pertussis Strains Circulating in Europe in 1999 to 2004 as Determined by Pulsed-Field Gel Electrophoresis
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Bordetella pertussis Strains Circulating in Europe in 1999 to 2004 as Determined by Pulsed-Field Gel Electrophoresis

机译：脉冲场凝胶电泳确定百日咳博德特氏菌在1999年至2004年间在欧洲流通

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Clinical isolates of Bordetella pertussis collected during the year 2004 (n = 153) in eight European countries, Denmark, Finland, France, Germany, The Netherlands, Poland, Sweden, and United Kingdom, were analyzed by pulsed-field gel electrophoresis (PFGE), and their PFGE profiles were compared with those of isolates collected in 1999 (n = 102). The 255 isolates produced 59 distinct PFGE profiles. Among the 153 isolates from 2004, 36 profiles were found, while within the 102 isolates from 1999, 33 profiles were detected. One PFGE profile, BpSR11, was dominant (30% to 50%) in all countries except Denmark (10%) and Poland (0%). In comparison with 1999, there was an increase in BpSR11 prevalence in Finland in 2004 from 5% to 40%, coinciding with a major incidence peak. Some other PFGE profiles seemed to be associated with limited dissemination. Poland was the only country in which the most common actual European PFGE profiles were not found. In a dendrogram analysis, all common PFGE profiles were identified within PFGE group IV, and BpSR11 clustered together with PFGE subgroup IVβ. Compared to the 1999 isolates, PFGE group V representative for pertactin variant prn3 strains had disappeared, and a new cluster was seen. In conclusion, some PFGE profiles, such as BpSR11, evidently have a higher capacity to spread, suggesting increased fitness to the present immunological environment. It is therefore of major interest to continue with surveillance programs of B. pertussis isolates, as both waning vaccine-derived immunity and strain variation may play a role in the persistence of pertussis.
机译：2004年（ n = 153）在八个欧洲国家，丹麦，芬兰，法国，德国，荷兰，波兰，瑞典和通过脉冲场凝胶电泳（PFGE）对英国进行了分析，并将其PFGE图谱与1999年收集的分离株（ n = 102）进行了比较。 255个分离物产生了59个不同的PFGE图谱。在2004年的153个分离株中，发现了36​​个图谱，而1999年的102个分离株中，发现了33个图谱。除丹麦（10％）和波兰（0％）外，在所有国家中，PFGE的一个特征是BpSR11，占主导地位（30％至50％）。与1999年相比，2004年芬兰的BpSR11患病率从5％增加到40％，这与主要的发病高峰相吻合。其他一些PFGE配置文件似乎与有限的传播有关。波兰是唯一未找到最常见的欧洲PFGE实际情况的国家。在树状图分析中，在PFGE组IV中确定了所有常见的PFGE图谱，并且BpSR11与PFGE亚组IVβ聚集在一起。与1999年的分离株相比，代表百日咳杆菌变蛋白 prn3 株的PFGE V组消失了，并且看到了一个新的簇。总之，某些PFGE图谱，例如BpSR11，显然具有更高的扩散能力，表明对当前免疫环境的适应性增强。因此，继续进行B的监视程序非常重要。百日咳杆菌分离株，因为疫苗的免疫力下降和菌株变异可能在百日咳的持久性中起作用。

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