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首页> 外文期刊>Journal of Clinical Microbiology >Evaluation of Different PCR Assays for Early Detection of Acute and Relapsing Brucellosis in Humans in Comparison with Conventional Methods
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Evaluation of Different PCR Assays for Early Detection of Acute and Relapsing Brucellosis in Humans in Comparison with Conventional Methods

机译：与常规方法比较，对早期检测人类急性和复发性布鲁氏菌病的不同PCR方法的评价

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Human brucellosis is a significant public health problem in many Mediterranean countries including Greece. The conventional serological methods, as well as blood cultures, have serious limitations, especially in chronic, relapsing, and focal forms of the disease. Four different PCR assays were applied in 4,926 buffy coat, whole-blood, and serum samples received from 200 patients admitted with brucellosis to the Infectious Diseases Hospital, Thessaloniki, Greece, for the rapid diagnosis of acute infection and relapses and compared to blood culture and serological tests (i.e., Wright's seroagglutination test, Coombs' antibrucella test, and the complement fixation test). The four PCR assays had excellent sensitivity and specificity and were able to detect all of the cases of acute disease. Buffy coat and whole blood were the optimal specimens. All four PCR assays were negative in all follow-up samples from 183 patients who had completed a successful treatment and were positive in all follow-up samples from 17 patients who had relapses in the first year after therapy, including the times of the relapses. In conclusion, PCR is a very useful tool for the rapid diagnosis of acute brucellosis and a good marker for the posttreatment follow-up and the early detection of relapses.
机译：在包括希腊在内的许多地中海国家中，人类布鲁氏菌病是一个重大的公共卫生问题。常规的血清学方法以及血液培养具有严重的局限性，特别是在该疾病的慢性，复发性和局灶性形式中。在希腊萨洛尼卡市传染病医院对200例布鲁氏菌病患者的4926例血沉棕黄层，全血和血清样本进行了四种不同的PCR检测，以快速诊断急性感染和复发，并与血培养和血清学检查（即赖特氏血清凝集试验，库姆斯抗小球藻试验和补体固定试验）。四种PCR检测法均具有出色的灵敏度和特异性，能够检测所有急性疾病病例。血沉棕黄层和全血是最佳标本。在完成成功治疗的183例患者的所有随访样本中，所有四种PCR检测均阴性，而在治疗后第一年复发的17例患者的所有随访样本中，所有4份PCR检测均为阳性，包括复发次数。总之，PCR是用于快速诊断急性布鲁氏菌病的非常有用的工具，并且是治疗后随访和早期发现复发的良好标志。

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