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首页> 外文期刊>Journal of Clinical Microbiology >Heteroresistance to Meropenem in Carbapenem-Susceptible Acinetobacter baumannii
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Heteroresistance to Meropenem in Carbapenem-Susceptible Acinetobacter baumannii

机译：碳青霉烯易感鲍曼不动杆菌对美罗培南的异抗性

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The characteristics of carbapenem heteroresistance were studied in 14 apparently carbapenem-susceptible Acinetobacter baumannii isolates. The MICs for carbapenems were determined, and the isolates were genotyped by pulsed-field gel electrophoresis (PFGE) and sequence typing (ST). Population analysis, testing of the stability of the heteroresistant subpopulations, and time-killing assays were performed. The agar dilution MICs of both imipenem and meropenem for the native isolates ranged from 0.25 to 4 mg/liter. The isolates belonged to nine PFGE types and exhibited seven ST allelic profiles. Population analysis revealed subpopulations that grew in the presence of imipenem at concentrations of up to 8 mg/liter and meropenem at concentrations of up to 32 mg/liter. The meropenem-heteroresistant subpopulations of 11 isolates exhibited stable resistance with MICs that ranged from 16 to >32 mg/liter; their PFGE profiles were identical to those of the native isolates. Time-kill assays with meropenem revealed less pronounced killing for 10 isolates. These findings indicate that meropenem pressure can produce meropenem-heteroresistant subpopulations that might subsequently select for highly resistant strains.
机译：在14个明显易感碳青霉烯的鲍曼不动杆菌中分离出碳青霉烯异抗性的特征。确定了碳青霉烯的MIC，并通过脉冲场凝胶电泳（PFGE）和序列分型（ST）对分离株进行了基因分型。进行了种群分析，杂合抗性亚群的稳定性测试和杀灭时间的测定。亚胺培南和美洛培南的琼脂稀释MIC对天然分离物的变化范围为0.25至4 mg / L。分离株属于9种PFGE类型，表现出7种ST等位基因特征。人口分析显示，亚胺培南存在时，亚群的浓度高达8毫克/升，而美洛培南的亚群则高达32毫克/升。 11个分离株的美罗培南-异抗性亚群显示出稳定的耐药性，MIC范围从16到> 32 mg / L。它们的PFGE谱与天然分离株的PFGE谱相同。美罗培南的时间杀灭测定显示10种分离株的杀灭作用不明显。这些发现表明美洛培南压力可以产生美洛培南-异抗性亚群，随后可能会选择高抗性菌株。

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