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首页> 外文期刊>Journal of Clinical Microbiology >Rapid Detection of Staphylococcus aureus Panton-Valentine Leukocidin in Clinical Specimens by Enzyme-Linked Immunosorbent Assay and Immunochromatographic Tests
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Rapid Detection of Staphylococcus aureus Panton-Valentine Leukocidin in Clinical Specimens by Enzyme-Linked Immunosorbent Assay and Immunochromatographic Tests

机译：酶联免疫吸附法和免疫色谱法快速检测临床标本中的金黄色葡萄球菌潘通-华伦特白蛋白

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Staphylococcus aureus strains producing Panton-Valentine leukocidin (PVL) have been epidemiologically linked to specific human infections. To evaluate immunological tests that may be used to diagnose infections with PVL-producing strains, we prospectively collected pus, respiratory tract specimens, and joint fluid specimens from which S. aureus had been isolated in clinical laboratories in six countries. An enzyme-linked immunosorbent assay (ELISA) and an immunochromatographic test (ICT) targeting LukS-PV were performed directly with clinical samples for the detection of PVL. The same tests were applied to S. aureus culture supernatants. The corresponding S. aureus isolates were characterized by PCR for the presence of the PVL locus (lukS-PV and lukF-PV) and the mecA gene. A total of 185 samples from 144 skin infections, 23 bone and joint infections, and 18 lower respiratory tract infections were analyzed. By PCR, 72/185 S. aureus isolates were PVL locus positive (PVL+); 28 of these were also mecA positive. PVL was detected in the supernatants of all PVL+ strains by both ELISA and an ICT, while no signal was observed with PVL-negative strains. The PVL concentrations in human clinical samples that grew PVL+ strains ranged from 0 to 399 μg/ml by ELISA. By the use of 0.015 μg/ml of PVL as a cutoff value, PVL was detected in 65/72 (90%) of the clinical samples by ELISA. The sensitivity and specificity of the ELISA test were 90% and 100%, respectively. By the ICT, PVL was detected in 57/72 (79%) of the samples, and the sensitivity and specificity of ICT were 79% and 100%, respectively. PVL is expressed by S. aureus during human infection, and a PVL-specific ELISA and ICT could be reliable tests for the diagnosis of infections caused by PVL-producing strains.
机译：在流行病学上，产生潘通-华伦特白蛋白（PVL）的金黄色葡萄球菌菌株与特定的人类感染有关。为了评估可用于诊断PVL产生菌株感染的免疫学测试，我们前瞻性地收集了脓，S呼吸道标本和关节液标本。在六个国家的临床实验室中已分离出金黄色葡萄球菌。直接针对临床样本进行针对LukS-PV的酶联免疫吸附测定（ELISA）和免疫色谱测试（ICT），以检测PVL。相同的测试应用于 S。金黄色葡萄球菌培养上清液。相应的 S。通过PCR鉴定金黄色葡萄球菌的PVL基因座（ lukS-PV 和 lukF-PV ）和 mec A 基因。共分析了144个皮肤感染，23个骨骼和关节感染以及18个下呼吸道感染的185个样本。通过PCR，72/185 S。金黄色葡萄球菌的分离株均为PVL基因阳性（PVL + ）;其中28个也是 mecA 阳性。通过ELISA和ICT在所有PVL + 菌株的上清液中检测到PVL，而PVL阴性菌株未观察到信号。通过ELISA，生长PVL + 菌株的人类临床样品中的PVL浓度范围为0至399μg/ ml。通过使用0.015μg/ ml的PVL作为临界值，通过ELISA在65/72（90％）的临床样品中检测到PVL。 ELISA检测的灵敏度和特异性分别为90％和100％。通过ICT，在57/72（79％）样本中检测到PVL，ICT的敏感性和特异性分别为79％和100％。 PVL用 S表示。人感染期间的金黄色葡萄球菌，以及PVL特异性ELISA和ICT可能是诊断由产生PVL的菌株引起的感染的可靠测试。

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