...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Detection of a Knockdown Resistance Mutation Associated with Permethrin Resistance in the Body Louse Pediculus humanus corporis by Use of Melting Curve Analysis Genotyping
【24h】

Detection of a Knockdown Resistance Mutation Associated with Permethrin Resistance in the Body Louse Pediculus humanus corporis by Use of Melting Curve Analysis Genotyping

机译：融化曲线分析基因分型技术检测人虱病中与氯菊酯抗性相关的抗击倒突变

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Louse-borne diseases are prevalent in the homeless, and body louse eradication has thus far been unsuccessful in this population. We aim to develop a rapid and robust genotyping method usable in large field-based clinical studies to monitor permethrin resistance in the human body louse Pediculus humanus corporis. We assessed a melting curve analysis genotyping method based on real-time PCR using hybridization probes to detect the M815I-T917I-L920F knockdown resistance (kdr) mutation in the paraorthologous voltage-sensitive sodium channel (VSSC) α subunit gene, which is associated with permethrin resistance. The 908-bp DNA fragment of the VSSC gene, encoding the α subunit of the sodium channel and encompassing the three mutation sites, was PCR sequenced from 65 lice collected from a homeless population. We noted a high prevalence of the 3 indicated mutations in the body lice collected from homeless people (100% for the M815I and L920F mutations and 56.73% for the T917I mutation). These results were confirmed by melting curve analysis genotyping, which had a calculated sensitivity of 100% for the M815I and T917I mutations and of 98% for the L920F mutation. The specificity was 100% for M815I and L920F and 96% for T917I. Melting curve analysis genotyping is a fast, sensitive, and specific tool that is fully compatible with the analysis of a large number of samples in epidemiological surveys, allowing the simultaneous genotyping of 96 samples in just over an hour (75 min). Thus, it is perfectly suited for the epidemiological monitoring of permethrin resistance in human body lice in large-scale clinical studies.
机译：虱子传播的疾病在无家可归者中很普遍，到目前为止，消灭虱子的方法在该人群中还没有成功。我们旨在开发一种快速，可靠的基因分型方法，可用于基于大领域的临床研究中，以监测人体虱子鼠疫中的苄氯菊酯抗性。我们评估了基于实时PCR的融化曲线分析基因分型方法，该杂交方法使用杂交探针来检测直系同源电压敏感钠通道（VSSC）α亚基基因中的M815I-T917I-L920F敲低抗性（kdr）突变氯菊酯抗性。从收集自无家可归者的65虱子中对VSSC基因的908 bp DNA片段进行PCR测序，该片段编码钠通道的α亚基，并包含三个突变位点。我们注意到从无家可归的人那里收集到的3种指示突变在虱子中普遍存在（M815I和L920F突变为100％，T917I突变为56.73％）。这些结果通过熔解曲线分析基因分型得到了证实，该基因分型对M815I和T917I突变具有100％的计算灵敏度，对L920F突变具有98％的计算灵敏度。 M815I和L920F的特异性为100％，T917I的特异性为96％。熔解曲线分析基因分型是一种快速，灵敏且特定的工具，与流行病学调查中大量样品的分析完全兼容，可在一个多小时（75分钟）内同时对96个样品进行基因分型。因此，它非常适合在大规模临床研究中对人体虱子中氯菊酯抗药性的流行病学监测。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号