PCR Amplification of the IS6110 Insertion Element of Mycobacterium tuberculosis in Fecal Samples from Patients with Intestinal Tuberculosis

Ramadass Balamurugan; Subramanian Venkataraman; K. R. John; Balakrishnan S. Ramakrishna

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PCR Amplification of the IS6110 Insertion Element of Mycobacterium tuberculosis in Fecal Samples from Patients with Intestinal Tuberculosis

机译：肠道结核患者粪便样本中结核分枝杆菌IS6110插入元件的PCR扩增

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PCR amplification of insertion element IS6110 of Mycobacterium tuberculosis in fecal samples was evaluated in the diagnosis of intestinal tuberculosis (ITB). The numbers of samples that tested positive by PCR with SalI digestion were 16/18 untreated-ITB samples, 0/8 treated-ITB samples, 12/14 smear-positive pulmonary tuberculosis samples, and 0/30 control samples. The sensitivity, specificity, positive predictive value, and negative predictive value of fecal PCR were 88.8%, 100%, 100%, and 93.7%, respectively.

机译：通过粪便样本中结核分枝杆菌的插入元件IS 6110 的PCR扩增，对肠道结核（ITB）的诊断进行了评估。通过SalI消化进行PCR检测呈阳性的样品数量为16/18未处理的ITB样品，0/8处理的ITB样品，12/14涂片阳性肺结核样品和0/30对照样品。粪便PCR的灵敏度，特异性，阳性预测值和阴性预测值分别为88.8％，100％，100％和93.7％。 展开▼

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《Journal of Clinical Microbiology》 |2006年第5期|共3页

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Ramadass Balamurugan; Subramanian Venkataraman; K. R. John; Balakrishnan S. Ramakrishna;
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中图分类医学微生物学（病原细菌学、病原微生物学）;

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1. PCR Amplification of the IS6110 Insertion Element of Mycobacterium tuberculosis in Fecal Samples from Patients with Intestinal Tuberculosis [J] . Ramadass Balamurugan, Subramanian Venkataraman, K. R. John, Journal of Clinical Microbiology . 2006,第5期

机译：肠道结核患者粪便样本中结核分枝杆菌IS6110插入元件的PCR扩增

2. The usefulness of PCR amplification of the IS6110 insertion element of M. tuberculosis complex in ascitic fluid of patients with peritoneal tuberculosis. [J] . Tzoanopoulos D, Giaglis S, Kartalis G European journal of internal medicine . 2003,第6期

机译：PCR扩增腹膜结核患者腹水中结核分枝杆菌复合物IS6110插入元件的有用性。

3. Fecal polymerase chain reaction for Mycobacterium tuberculosis IS6110 to distinguish Crohn’s disease from intestinal tuberculosis [J] . Balamurugan Ramadass, Sucharita Chittaranjan, Venkataraman Subramanian, Indian journal of gastroenterology . 2010,第July期

机译：粪便聚合酶链反应用于结核分枝杆菌IS6110，以区分克罗恩病和肠道结核

4. Quantitative Real-Time PCR for the detection of Mycobacterium avium subsp. paratuberculosis in bovine fecal samples and liquid culture samples [C] . Sweeney RW, Mangold BL, McAdams S, International Colloquium on Paratuberculosis . 2007

机译：用于检测分枝杆菌亚空间的定量实时PCR。牛粪样品和液体培养样品的副植物

5. Drug Resistance in Mycobacterium tuberculosis: An Investigation of Drug Transporter P-Glycoprotein and an Evaluation of Improved Diagnosis of Drug Resistant Tuberculosis in Cameroonian Pulmonary Tuberculosis Patients [D] . Abanda, Ngu Njei 2017

机译：结核分枝杆菌的耐药性：转运蛋白P-糖蛋白的调查和喀麦隆肺结核患者耐药性结核病改善诊断的评估

6. PCR Amplification of the IS6110 Insertion Element of Mycobacterium tuberculosis in Fecal Samples from Patients with Intestinal Tuberculosis [O] . Ramadass Balamurugan, Subramanian Venkataraman, K. R. John, 2006

机译：肠道结核患者粪便样本中结核分枝杆菌IS6110插入元件的PCR扩增

7. PCR Amplification of the IS6110 Insertion Element of Mycobacterium tuberculosis in Fecal Samples from Patients with Intestinal Tuberculosis [O] . Balamurugan, Ramadass, Venkataraman, Subramanian, John, K. R., 2006

机译：肠道结核患者粪便样本中结核分枝杆菌IS6110插入元件的PCR扩增

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3. PCR扩增IS6110检测结核分枝杆菌的特异性和可靠性 [J] . 杨朝国 ,罗涛 . 四川省卫生管理干部学院学报 . 1997,第004期

4. PCR扩增IS6110在结核病检测和流行病学中的应用评价 [J] . 赵自云 ,朱玲 . 中国人兽共患病学报 . 2008,第003期

5. DNA微阵列芯片技术检测培阳肺结核患者痰样本中结核分枝杆菌耐药性的价值 [J] . 王悦 ,孙颖 ,孙炳奇 . 中国人兽共患病学报 . 2021,第002期

6. 测序分型中2例样本HLA-DQB1等位基因第2外显子PCR扩增丢失及其原因分析 [C] . Gao Su Qing ,高素青 ,Wang Daming . 中华骨髓库第四届年会 . 2013

7. 临床感染样本和粪便调查样本中耐碳青霉烯类肠杆菌科细菌的分子特征研究 [A] . 段小女 . 2020

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2. 一种人类粪便肠道菌群样本的处理方法及处理试剂 [P] . 中国专利： CN111534466B . 2021.06.04

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PCR Amplification of the IS6110 Insertion Element of Mycobacterium tuberculosis in Fecal Samples from Patients with Intestinal Tuberculosis

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