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首页> 外文期刊>Journal of Clinical Microbiology >Monoclonal Antibodies to VP1 Recognize a Broad Range of Enteroviruses
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Monoclonal Antibodies to VP1 Recognize a Broad Range of Enteroviruses

机译：VP1单克隆抗体可识别多种肠道病毒

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Enteroviruses (EVs) are common seasonal viruses that are associated with a variety of diseases. High-quality monoclonal antibodies (MAbs) are needed to improve the accuracy of EV diagnosis in clinical laboratories. In the present study, the full-length VP1 genes of poliovirus 1 (Polio 1) and coxsackievirus B3 (Cox B3) were cloned, and the encoded proteins were expressed and used as antigens in an attempt to raise broad-spectrum MAbs to EVs. Two pan-EV MAbs were isolated: one raised against Polio 1 VP1 and the other against Cox B3 VP1. The binding sites of both pan-EV MAbs were mapped to an amino acid sequence within a conserved region in the N terminus of Polio 1 VP1 by peptide and competition enzyme-linked immunosorbent assay. Two additional MAbs, an EV70-specific MAb and an EV71/Cox A16-bispecific MAb, developed against EV70 and 71 VP1 proteins, were pooled with the two pan-EV MAbs (pan-EV MAb mix) and tested for their sensitivity and specificity in the staining of various virus-infected cells. The pan-EV MAb mix detected all 40 prototype EVs tested and showed no cross-reactivity to 18 different non-EV human viruses. Compared with two commercially available EV tests, the pan-EV MAb mix exhibited higher specificity than one test and broader spectrum reactivity than the other. Thus, our study demonstrates that full-length Polio 1 VP1 and Cox B3 VP1 can serve as effective antigens for developing a pan-EV MAb and that the pan-EV MAb mix can be used for the laboratory diagnosis of a wide range of EV infections.
机译：肠病毒（EVs）是常见的季节性病毒，与多种疾病有关。需要高质量的单克隆抗体（MAb）来提高临床实验室中EV诊断的准确性。在本研究中，克隆了脊髓灰质炎病毒1（脊髓灰质炎1）和柯萨奇病毒B3（Cox B3）的全长VP1基因，并表达了编码的蛋白并将其用作抗原，以尝试将广谱MAb提升至EV。分离出两个pan-EV MAb：一个针对脊髓灰质炎1 VP1，另一个针对Cox B3 VP1。通过肽和竞争酶联免疫吸附测定，将两​​个pan-EV MAb的结合位点定位到脊髓灰质炎1VP1的N末端保守区域内的氨基酸序列。针对这两种EV70和71 VP1蛋白开发的另外两种MAb，即EV70特异性MAb和EV71 / Cox A16双特异性MAb，与两种泛EV MAb（泛EV MAb混合物）合并，并对其敏感性和特异性进行了测试。在各种病毒感染的细胞染色中pan-EV MAb混合物可检测到所有40种原型EV，并且与18种不同的非EV人类病毒无交叉反应。与两种市售的EV测试相比，pan-EV MAb混合物显示出比一种测试更高的特异性，并且比另一种更高的光谱反应性。因此，我们的研究表明全长脊髓灰质炎1 VP1和Cox B3 VP1可以作为开发pan-EV MAb的有效抗原，并且pan-EV MAb混合物可用于实验室诊断广泛的EV感染。

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