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首页> 外文期刊>Journal of Clinical Microbiology >Molecular Surveillance of Clinical Neisseria gonorrhoeae Isolates in Russia
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Molecular Surveillance of Clinical Neisseria gonorrhoeae Isolates in Russia

机译：俄罗斯临床淋病奈瑟菌分离株的分子监测

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The choice of adequate methods for epidemiological purposes remains a challenging problem in Neisseria gonorrhoeae molecular monitoring. In this study, the collection of geographically unrelated gonococci (n = 103) isolated in Russian clinics was comparably tested by (i) a traditional serotyping scheme, (ii) por typing, (iii) Neisseria gonorrhoeae multiantigen sequence typing (NG-MAST), and (iv) multilocus sequence typing (MLST). It is shown that, according to sequencing data, a third of the strains carried new porB1 alleles, as well as tbpB ones, and more than half of the samples had new sequence types (STs) as determined by NG-MAST or MLST. The discriminatory power for each typing method was calculated by using the Hunter-Gaston discriminatory index, D. Commonly, modern nucleic acid-based typing methods (por typing, NG-MAST, and MLST) appeared to be more efficient than the classical serotyping scheme. While the traditional serotyping gave a D value of 0.82, the por typing, NG-MAST, and MLST approaches yielded D values of 0.97, 0.98, and 0.91, respectively. Each typing technique revealed the distribution of gonococci slightly correlated with their geographical sources. However, only the MLST method STs were highly associated with certain phenotypes. Although ST1594, ST1892, and ST6720 were typical for susceptible gonococci, ST1901 and ST6716 were undoubtedly associated with a multidrug-resistant phenotype. We conclude that every tested nucleic acid-based typing method is suitable for N. gonorrhoeae molecular surveillance. However, the MLST method seems to serve large-scale epidemiological purposes, whereas the NG-MAST and por typing approaches are more appropriate for the investigation of local outbreaks.
机译：在淋病奈瑟菌分子监测中，为流行病学目的选择适当的方法仍然是一个具有挑战性的问题。在这项研究中，通过（i）传统的血清分型方案（ii） por 分型，比较了在俄罗斯诊所中分离的地理无关的淋球菌（ n = 103）的收集情况，（iii）淋病奈瑟氏球菌多抗原序列分型（NG-MAST）和（iv）多基因座序列分型（MLST）。结果表明，根据测序数据，三分之一的菌株携带了新的 porB1 等位基因，以及 tbpB 等位基因，超过一半的样品具有新的序列由NG-MAST或MLST确定的类型（ST）。使用Hunter-Gaston判别指标 D 计算每种打字方法的判别能力。通常，现代的基于核酸的分型方法（ por 分型，NG-MAST和MLST）似乎比传统的血清分型方法更有效。传统的血清分型法给出的 D 值为0.82，而 por 的分型，NG-MAST和MLST方法产生的 D 值分别为0.97、0.98 ，和0.91分别。每种打字技术都揭示了淋球菌的分布与其地理来源略有关联。但是，只有MLST方法ST与某些表型高度相关。尽管ST1594，ST1892和ST6720是易感性淋球菌的典型代表，但ST1901和ST6716无疑与耐多药表型有关。我们得出结论，每种经过测试的基于核酸的分型方法均适用于 N。淋病分子监测。但是，MLST方法似乎可用于大规模流行病学目的，而NG-MAST和 por 分型方法更适合于调查当地的暴发流行。

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