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Photo-inactivation of Neisseria gonorrhoeae: A paradigm changing approach for combating antibiotic-resistant gonococcal infection

机译:淋病奈瑟氏球菌的光灭活:对抗耐药性淋球菌感染的范式改变方法

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Antimicrobial resistance in Neisseria gonorrhoeae is a major issue of public health, and there is a critical need for thedevelopment of new anti-gonococcal strategies. In this study, we investigated the effectiveness of antimicrobial bluelight (aBL; 405 nm wavelength), an innovative non-pharmacological approach, for the inactivation of N. gonorrhoeae.Our findings indicated that aBL preferentially inactivated N. gonorrhoeae, including antibiotic-resistant strains, overhuman vaginal epithelial cells in vitro. Furthermore, no genotoxicity of aBL to the vaginal epithelial cells was observedat the radiant exposure for inactivating N. gonorrhoeae. aBL also effectively inactivated N. gonorrhoeae that had attachedto and invaded into the vaginal epithelial cells in their co-cultures. No gonococcal resistance to aBL developedafter 15 successive cycles of sub-therapeutic aBL inactivation. Taken together, aBL represents a potent potential treatmentfor antibiotic-resistant gonococcal infection.
机译:淋病奈瑟氏球菌的抗药性是公共卫生的一个重要问题,因此迫切需要开发新的抗性淋球菌策略。在这项研究中,我们研究了一种创新的非药理方法抗菌蓝\ n \ nlight(aBL; 405 nm波长)对淋病奈瑟氏球菌的灭活效果。\ r \ n我们的发现表明,aBL优先灭活了淋病奈瑟氏球菌。淋球菌,包括抗生素抗性菌株,在体外超过人的阴道上皮细胞。此外,没有观察到aBL对阴道上皮细胞的遗传毒性/没有辐射到淋病奈瑟氏球菌的灭活。 aBL还可以有效地灭活淋病奈瑟氏球菌,该菌在它们的共培养物中已附着并侵袭到阴道上皮细胞中。在亚治疗性aBL失活的连续15个周期后,未出现对aBL的淋球菌耐药性。综上所述,aBL代表了对抗生素耐药的淋球菌感染的潜在潜在治疗方法。

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    Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School,Boston, MA, USA 02114 Department of Laser Medicine, Chinese PLA General Hospital, Beijing,China 100853;

    Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School,Boston, MA, USA 02114 Vaccine and Immunotherapy Center, Massachusetts General Hospital, HarvardMedical School, Boston, Massachusetts, USA;

    Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School,Boston, MA, USA 02114;

    Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School,Boston, MA, USA 02114 Vaccine and Immunotherapy Center, Massachusetts General Hospital, HarvardMedical School, Boston, Massachusetts, USA;

    Department of Radiation Oncology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA 02114;

    Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, MA, USA 02115;

    Department of Laser Medicine, Chinese PLA General Hospital, Beijing, China 100853;

    Vaccine and Immunotherapy Center, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA;

    Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School,Boston, MA, USA 02114 Vaccine and Immunotherapy Center, Massachusetts General Hospital, HarvardMedical School, Boston, Massachusetts, USA;

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