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首页> 外文期刊>Journal of Clinical Microbiology >Microbiome Diversity in the Bronchial Tracts of Patients with Chronic Obstructive Pulmonary Disease
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Microbiome Diversity in the Bronchial Tracts of Patients with Chronic Obstructive Pulmonary Disease

机译：慢性阻塞性肺疾病患者支气管中的微生物组多样性

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Culture of bacteria from bronchial secretions in respiratory patients has low sensitivity and does not allow for complete assessment of microbial diversity across different bronchial compartments. In addition, a significant number of clinical studies are based on sputum samples, and it is not known to what extent they describe the real diversity of the mucosa. In order to identify previously unrecognized lower airway bacteria and to investigate the complexity and distribution of microbiota in patients with chronic obstructive pulmonary disease (COPD), we performed PCR amplification and pyrosequencing of the 16S rRNA gene in patients not showing signs or symptoms of infection. Four types of respiratory samples (sputum, bronchial aspirate, bronchoalveolar lavage, and bronchial mucosa) were taken from each individual, obtaining on average >1,000 16S rRNA sequences per sample. The total number of genera per patient was >100, showing a high diversity, with Streptococcus, Prevotella, Moraxella, Haemophilus, Acinetobacter, Fusobacterium, and Neisseria being the most commonly identified. Sputum samples showed significantly lower diversity than the other three sample types. Lower-bronchial-tree samples, i.e., bronchoalveolar lavage and bronchial mucosa, showed a very similar bacterial compositions in contrast to sputum and bronchial aspirate samples. Thus, sputum and bronchial aspirate samples are upper bronchial tree samples that are not representative of the lower bronchial mucosa flora, and bronchoalveolar lavage samples showed the results closest to those for the bronchial mucosa. Our data confirm that the bronchial tree is not sterile in COPD patients and support the existence a different microbiota in the upper and lower compartments.
机译：呼吸道患者支气管分泌物中细菌的培养敏感性低，无法全面评估不同支气管腔室之间的微生物多样性。另外，大量的临床研究都是基于痰液样本，尚不清楚它们在多大程度上描述了粘膜的真正多样性。为了鉴定先前无法识别的下呼吸道细菌并调查慢性阻塞性肺疾病（COPD）患者中微生物群的复杂性和分布，我们对未显示感染迹象或症状的患者进行了16S rRNA基因的PCR扩增和焦磷酸测序。从每个个体中提取四种呼吸样品（痰，支气管抽吸液，支气管肺泡灌洗液和支气管粘膜），每个样品平均获得> 1,000个16S rRNA序列。每位患者的总属数> 100，显示出高度的多样性，其中最常见的是链球菌，普氏菌，莫拉氏菌，嗜血杆菌，不动杆菌，梭菌和奈瑟氏球菌。痰液样本显示的多样性明显低于其他三种样本类型。下支气管树样品，即支气管肺泡灌洗液和支气管粘膜，与痰液和支气管抽吸物样品相比，细菌组成非常相似。因此，痰和支气管抽吸物样品是上支气管树样品，不代表下支气管粘膜菌群，支气管肺泡灌洗样品显示的结果最接近于支气管粘膜。我们的数据证实，COPD患者的支气管树不是无菌的，并支持上下隔室中存在不同的微生物群。

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