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首页> 外文期刊>Journal of Clinical Microbiology >Malassezia arunalokei sp. nov., a Novel Yeast Species Isolated from Seborrheic Dermatitis Patients and Healthy Individuals from India
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Malassezia arunalokei sp. nov., a Novel Yeast Species Isolated from Seborrheic Dermatitis Patients and Healthy Individuals from India

机译:马拉色马拉色菌nov。,一种分离自脂溢性皮炎患者和印度健康个体的新型酵母菌

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The majority of species within the genus Malassezia are lipophilic yeasts that colonize the skin of warm-blooded animals. Two species, Malassezia globosa and Malassezia restricta, are implicated in the causation of seborrheic dermatitis/dandruff (SD/D). During our survey of SD/D cases, we isolated several species of Malassezia and noticed vast variations within a few lipid-dependent species. Variations observed in the phenotypic characteristics (colony morphology, absence of catalase activity, growth at 37°C, and precipitation surrounding wells containing Tween 20 or Cremophor EL) suggested the possible presence of a novel species. Sequence divergence observed in the internal transcribed spacer (ITS) region, the D1/D2 domain, and the intergenic spacer 1 (IGS1) region of rDNA and the TEF1 gene, PCR-restriction fragment length polymorphism (RFLP) analysis of the ITS2 region, and fluorescent amplified fragment length polymorphism analysis support the existence of a novel species. Based on phenotypic and molecular characterization of these strains, we propose a new species, namely, M. arunalokei sp. nov., and we designate NCCPF 127130 (= MTCC 12054 = CBS 13387) as the type strain.
机译:马拉色氏菌属中的大多数物种是亲脂性酵母,它们定植在温血动物的皮肤上。脂溢性马拉色氏菌和头皮屑的两个物种与脂溢性皮炎/头皮屑(SD / D)的病因有关。在我们对SD / D病例的调查中,我们分离出了数个马拉色菌属物种,并发现了一些脂质依赖性物种内的巨大差异。表型特征的变化(菌落形态,过氧化氢酶活性的缺失,37°C下的生长以及含有Tween 20或Cremophor EL的孔周围的沉淀)表明可能存在新物种。在rDNA和 TEF1 基因的内部转录间隔区(ITS),D1 / D2结构域和基因间隔区1(IGS1)区中观察到序列差异,PCR限制性片段长度多态性(RFLP) )的ITS2区域分析,以及荧光扩增的片段长度多态性分析支持了新物种的存在。基于这些菌株的表型和分子特征,我们提出了一个新种,即M. arunalokei sp。 11月,我们将NCCPF 127130(= MTCC 12054 = CBS 13387)指定为类型应变。

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