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首页> 外文期刊>Journal of Clinical Microbiology >Identification of Medically Relevant Species of Arthroconidial Yeasts by Use of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
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Identification of Medically Relevant Species of Arthroconidial Yeasts by Use of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

机译：通过基质辅助激光解吸电离-飞行时间质谱法鉴定医学上与节肢动物酵母有关的物种

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Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) was used for an extensive identification study of arthroconidial yeasts, using 85 reference strains from the CBS-KNAW yeast collection and 134 clinical isolates collected from medical centers in Qatar, Greece, and Romania. The test set included 72 strains of ascomycetous yeasts (Galactomyces, Geotrichum, Saprochaete, and Magnusiomyces spp.) and 147 strains of basidiomycetous yeasts (Trichosporon and Guehomyces spp.). With minimal preparation time, MALDI-TOF MS proved to be an excellent diagnostic tool that provided reliable identification of most (98%) of the tested strains to the species level, with good discriminatory power. The majority of strains were correctly identified at the species level with good scores (>2.0) and seven of the tested strains with log score values between 1.7 and 2.0. The MALDI-TOF MS results obtained were consistent with validated internal transcribed spacer (ITS) and/or large subunit (LSU) ribosomal DNA sequencing results. Expanding the mass spectrum database by increasing the number of reference strains for closely related species, including those of nonclinical origin, should enhance the usefulness of MALDI-TOF MS-based diagnostic analysis of these arthroconidial fungi in medical and other laboratories.
机译：基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）被用于关节孢子酵母的广泛鉴定研究，使用了来自CBS-KNAW酵母收集物中的85个参考菌株和从卡塔尔医疗中心收集的134种临床分离株，希腊和罗马尼亚。该测试集包括72株子囊酵母（乳杆菌，大肠菌，腐生菌和Magnusiomyces spp。）和147副担子菌酵母（Trichosporon和Guehomyces spp。）。 MALDI-TOF MS用最短的准备时间被证明是一种出色的诊断工具，可对大多数（98％）被测菌株进行可靠的鉴定，并具有良好的区分能力。在品系水平上，大多数菌株已正确鉴定，得分较高（> 2.0），对数测试菌株中有7株的对数值在1.7和2.0之间。获得的MALDI-TOF MS结果与经过验证的内部转录间隔区（ITS）和/或大亚基（LSU）核糖体DNA测序结果一致。通过增加密切相关物种（包括非临床起源物种）的参考菌株的数量来扩展质谱数据库，应在医学和其他实验室中增强基于MALDI-TOF MS的这些节肢孢子真菌诊断分析的实用性。

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