...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Genomic Epidemiology and Molecular Resistance Mechanisms of Azithromycin-Resistant Neisseria gonorrhoeae in Canada from 1997 to 2014
【24h】

Genomic Epidemiology and Molecular Resistance Mechanisms of Azithromycin-Resistant Neisseria gonorrhoeae in Canada from 1997 to 2014

机译：1997年至2014年加拿大耐阿奇霉素淋病奈瑟菌的基因组流行病学和分子耐药机制

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The emergence of Neisseria gonorrhoeae strains with decreased susceptibility to cephalosporins and azithromycin (AZM) resistance (AZMr) represents a public health threat of untreatable gonorrhea infections. Genomic epidemiology through whole-genome sequencing was used to describe the emergence, dissemination, and spread of AZMr strains. The genomes of 213 AZMr and 23 AZM-susceptible N. gonorrhoeae isolates collected in Canada from 1989 to 2014 were sequenced. Core single nucleotide polymorphism (SNP) phylogenomic analysis resolved 246 isolates into 13 lineages. High-level AZMr (MICs ≥ 256 μg/ml) was found in 5 phylogenetically diverse isolates, all of which possessed the A2059G mutation (Escherichia coli numbering) in all four 23S rRNA alleles. One isolate with high-level AZMr collected in 2009 concurrently had decreased susceptibility to ceftriaxone (MIC = 0.125 μg/ml). An increase in the number of 23S rRNA alleles with the C2611T mutations (E. coli numbering) conferred low to moderate levels of AZMr (MICs = 2 to 4 and 8 to 32 μg/ml, respectively). Low-level AZMr was also associated with mtrR promoter mutations, including the ?35A deletion and the presence of Neisseria meningitidis-like sequences. Geographic and temporal phylogenetic clustering indicates that emergent AZMr strains arise independently and can then rapidly expand clonally in a region through local sexual networks.
机译：淋病奈瑟氏球菌菌株的出现，对头孢菌素和阿奇霉素（AZM）耐药（AZM r ）的敏感性降低，这代表了无法治愈的淋病感染的公共卫生威胁。通过全基因组测序的基因组流行病学描述了AZM r 菌株的出现，传播和传播。对1989年至2014年在加拿大收集的213株AZM r 和23株AZM易感性淋病奈瑟氏球菌的基因组进行了测序。核心单核苷酸多态性（SNP）系统遗传学分析将246个分离株分为13个谱系。在5个系统发育多样的分离物中发现了高水平的AZM r （MIC≥256μg/ ml），所有这些分离物在所有四个23S rRNA等位基因中均具有A2059G突变（大肠杆菌编号）。 2009年同时收集到的一株高水平AZM r 菌株对头孢曲松的敏感性降低（MIC = 0.125μg/ ml）。具有C2611T突变的23S rRNA等位基因数量的增加（大肠杆菌编号）赋予AZM r 低至中等水平（MIC分别为2至4和8至32μg/ ml） 。低水平的AZM r 也与 mtrR 启动子突变相关，包括？35A缺失和脑膜炎奈瑟氏球菌样序列的存在。地理和时间的系统发育聚类表明，出现的AZM r 菌株独立出现，然后可以通过局部性网络在一个区域内迅速克隆扩展。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号