...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Diagnosis of Mediterranean Visceral Leishmaniasis by Detection of Leishmania Antibodies and Leishmania DNA in Oral Fluid Samples Collected Using an Oracol Device
【24h】

Diagnosis of Mediterranean Visceral Leishmaniasis by Detection of Leishmania Antibodies and Leishmania DNA in Oral Fluid Samples Collected Using an Oracol Device

机译：通过检测使用Oracol装置收集的口腔液样品中的利什曼原虫抗体和利什曼原虫DNA诊断地中海内脏利什曼病

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Current methods for diagnosis of visceral leishmaniasis (VL) require invasive sampling procedures such as visceral aspiration and/or blood drawing. The use of diagnostic tests using oral fluid, which is easier to collect, would be more simple and practical for VL diagnosis, especially under field conditions. Oral fluids from 37 VL patients and 40 healthy controls were collected using Oracol devices. Blood samples and oral fluid specimens from both groups were analyzed by recombinant protein K39 (rK39) enzyme-linked immunosorbent assay and quantitative real-time PCR. Detection of antibodies in the oral fluid had a sensitivity of 100% and a specificity of 97.5%. Antibody levels measured in serum and oral fluid showed a significant positive correlation (ρ = 0.655 and P = 0.01). Detection of Leishmania DNA in oral fluid had a sensitivity of 94.6% and a specificity of 90%. The median parasite load estimated in blood was 133 parasites/ml (interquartile range [IR], 10 to 1,048), whereas that in oral fluid specimens was 3 parasites/ml (IR, 0.41 to 92). However, there was no significant linear relationship between parasite loads assessed in the two biological samples (ρ = 0.31 and P = 0.06). VL diagnosis based on specific antibody detection and Leishmania DNA identification using oral fluid samples was equivalent in accuracy to that using blood and therefore is promising for clinical use.
机译：当前诊断内脏利什曼病（VL）的方法需要侵入性采样程序，例如内脏抽吸和/或抽血。使用易于收集的口腔液进行诊断测试，对于VL诊断尤其是在野外条件下，将更加简单实用。使用Oracol装置收集了来自37例VL患者和40名健康对照的口服液。通过重组蛋白K39（rK39）酶联免疫吸附测定和定量实时PCR分析两组的血液样本和口腔液样本。口腔液中抗体的检测灵敏度为100％，特异性为97.5％。血清和口腔液中的抗体水平呈显着正相关（ρ= 0.655， P = 0.01）。口腔液中利什曼原虫DNA的检测灵敏度为94.6％，特异性为90％。估计的血液中寄生虫负荷中位数为133寄生虫/毫升（四分位间距[IR]，10至1,048），而口腔液标本中的寄生虫负荷为3寄生虫/毫升（IR，0.41至92）。但是，在两种生物样品中评估的寄生虫负荷之间没有显着的线性关系（ρ= 0.31， P = 0.06）。基于特定抗体检测和利什曼原虫DNA鉴定的VL诊断（使用口腔液样品）的准确性与使用血液的准确性相当，因此有望用于临床。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号