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首页> 外文期刊>Journal of Clinical Microbiology >Analysis of the Excretion Dynamics and Genotypic Characteristics of Rotavirus A during the Lives of Pigs Raised on Farms for Meat Production
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Analysis of the Excretion Dynamics and Genotypic Characteristics of Rotavirus A during the Lives of Pigs Raised on Farms for Meat Production

机译：产肉场养猪过程中轮状病毒A的排泄动态和基因型特征分析

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To determine the excretion dynamics and genotypic characteristics of rotavirus A (RVA), a longitudinal observational study was performed in 10 pigs from 3 litters at a farrow-to-finish farm. A total of 400 fecal samples were directly collected from the rectums of individual pigs (aged 7 to 217 days) at 3- to 14-day intervals. Seventy-one samples (17.5%) were positive for RVA by reverse transcription-PCR designed to detect the VP7 and VP4 genes. At least 13 combinations of 5 G (G2, G4, G5, G9, and G11) and 6 P (P[6], P[7], P[13], P[23], P[27], and P[34]) genotypes were identified by direct sequencing of the PCR products. We were able to detect RVA VP7 sequences from each pig 4 to 6 times with intervals of 7 to 52 days (from 7 to 119 days of age). Each pig harbored RVAs with at least 3 to 6 different combinations of G and P genotypes, while repeated excretions of RVAs carrying the same combinations of G and P genotypes were also observed. Virus shedding and changes in G and P genotypes appeared to be associated with movement of the pigs into weaning, growing, and finishing barns. These results indicated that, over their lifetimes, pigs raised for meat frequently and intermittently excrete genetically diverse RVAs.
机译：为了确定轮状病毒A（RVA）的排泄动态和基因型特征，在分娩至成年农场的3窝仔猪中对10头猪进行了纵向观察研究。以3至14天的间隔直接从个别猪（7至217天大）的直肠中采集总共400份粪便样品。通过设计用于检测VP7和VP4基因的逆转录PCR，有71个样品（17.5％）的RVA阳性。 5 G（G2，G4，G5，G9和G11）和6 P（P [6]，P [7]，P [13]，P [23]，P [27]和P的至少13种组合[34]）的基因型通过PCR产物的直接测序来鉴定。我们能够检测每只猪的RVA VP7序列4到6次，间隔为7到52天（从7到119天大）。每头猪的RVA具有至少3至6种不同的G和P基因型组合，同时还观察到RVA重复排泄，它们携带相同的G和P基因型组合。病毒的脱落以及G和P基因型的变化似乎与猪进入断奶，生长和肥育猪舍有关。这些结果表明，在猪的一生中，它们经常为肉而断断续续地排泄遗传多样性的RVA。

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