Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Meropenem Hydrolysis Assay with NH4HCO3, a Reliable Tool for Direct Detection of Carbapenemase Activity

Costas C. Papagiannitsis; Vendula ?tudentová; Radoslaw Izdebski; Olga Oikonomou; Yvonne Pfeifer; Efthimia Petinaki; Jaroslav Hrabák

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Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Meropenem Hydrolysis Assay with NH4HCO3, a Reliable Tool for Direct Detection of Carbapenemase Activity

机译：基质辅助激光解吸电离–飞行时间质谱NH4HCO3的美洛培南水解测定，一种直接检测碳青霉烯酶活性的可靠工具

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A comparison of a matrix-assisted laser desorption ionization–time of flight mass spectrometric (MALDI-TOF MS) meropenem hydrolysis assay with the Carba NP test showed that both methods exhibited low sensitivity (approximately 76%), mainly due to the false-negative results obtained with OXA-48-type producers. The addition of NH₄HCO₃ to the reaction buffer for the MALDI-TOF MS assay dramatically improved its sensitivity (98%). Automatic interpretation of the MALDI-TOF MS assay, using the MBT STAR-BL software, generally agreed with the results obtained after manual analysis. For the Carba NP test, spectrophotometric analysis found six additional carbapenemase producers.

机译：基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）美洛培南水解测定与Carba NP测试的比较表明，两种方法均显示出低灵敏度（约76％），这主要是由于假阴性用OXA-48型生产者获得的结果。在用于MALDI-TOF MS分析的反应缓冲液中添加NH _{4 HCO _{3 大大提高了其灵敏度（98％）。使用MBT STAR-BL软件自动解释MALDI-TOF MS分析，通常与手动分析后获得的结果一致。对于Carba NP测试，分光光度分析发现了另外6个碳青霉烯酶生产商。}}

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《Journal of Clinical Microbiology》 |2015年第5期|共5页
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Costas C. Papagiannitsis; Vendula ?tudentová; Radoslaw Izdebski; Olga Oikonomou; Yvonne Pfeifer; Efthimia Petinaki; Jaroslav Hrabák;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Meropenem Hydrolysis Assay with NH4HCO3, a Reliable Tool for Direct Detection of Carbapenemase Activity [J] . Costas C. Papagiannitsis, Vendula ?tudentová, Radoslaw Izdebski, Journal of Clinical Microbiology . 2015,第5期

机译：基质辅助激光解吸电离–飞行时间质谱NH4HCO3的美洛培南水解测定，一种直接检测碳青霉烯酶活性的可靠工具
2. Rapid Identification of Klebsiella pneumoniae by Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry and Detection of Meropenem Resistance by Flow Cytometric Assay [J] . Kilic Abdullah, Dogan Eyup, Kaya Sinem, Journal of clinical laboratory analysis. . 2016,第6期

机译：基质辅助激光解吸/电离-飞行时间质谱快速鉴定肺炎克雷伯氏菌和流式细胞术检测耐美罗培南
3. Comparison of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry assay with conventional methods for detection of IMP-6, VIM-2, NDM-1, SIM-1, KPC-1, OXA-23, and OXA-51 carbapenemase-producing Acinetobacter spp., Pseudomonas aeruginosa, and Klebsiella pneumoniae [J] . LeeW., ChungH.-S., LeeY., Diagnostic microbiology and infectious disease . 2013,第3期

机译：基质辅助激光解吸电离飞行时间质谱分析法与常规方法对IMP-6，VIM-2，NDM-1，SIM-1，KPC-1，OXA-23和OXA-的检测方法的比较51种产生碳青霉烯酶的不动杆菌属，铜绿假单胞菌和肺炎克雷伯菌
4. Optimization of Sample Preparation for Detection of Common Food-borne Pathogens by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry [C] . Lay Hoon Seah, Tze Horng Liew, Aldrich Leow, ASMS Conference on Mass Spectrometry and Allied Topics . 2015

机译：通过基质辅助激光解吸电离 - 飞行质谱法检测常见食品传播病原体的样品制剂的优化
5. Characterization of a specific single nucleotide polymorphism using limited primer extension assay and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. [D] . Schilling, George Leo, V. 2007

机译：使用有限的引物延伸测定和基质辅助激光解吸/电离飞行时间质谱分析特定单核苷酸多态性。
6. Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Meropenem Hydrolysis Assay with NH4HCO3 a Reliable Tool for Direct Detection of Carbapenemase Activity [O] . Costas C. Papagiannitsis, Vendula Študentová, Radoslaw Izdebski, 2015

机译：基质辅助激光解吸电离–飞行时间质谱NH4HCO3的美洛培南水解测定法一种直接检测碳青霉烯酶活性的可靠工具
7. Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Meropenem Hydrolysis Assay with NH4HCO3, a Reliable Tool for Direct Detection of Carbapenemase Activity [O] . Papagiannitsis, C. C., Studentova, V., Izdebski, R., 2015

机译：基质辅助激光解吸电离 - 飞行时间质谱法用NH4HCO3进行美罗培南水解测定，NH4HCO3是一种直接检测碳青霉烯酶活性的可靠工具

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Meropenem Hydrolysis Assay with NH4HCO3, a Reliable Tool for Direct Detection of Carbapenemase Activity

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