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首页> 外文期刊>Journal of Clinical Microbiology >Candida bracarensis Detected among Isolates of Candida glabrata by Peptide Nucleic Acid Fluorescence In Situ Hybridization: Susceptibility Data and Documentation of Presumed Infection
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Candida bracarensis Detected among Isolates of Candida glabrata by Peptide Nucleic Acid Fluorescence In Situ Hybridization: Susceptibility Data and Documentation of Presumed Infection

机译：肽核酸荧光原位杂交技术检测到光滑念珠菌分离株中的假丝酵母：敏感性数据和推测感染的文献

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Molecular taxonomic studies have revealed new Candida species among phenotypically delineated species, the best example being Candida dubliniensis. This study was designed to determine the occurrence of two new molecularly defined species, Candida bracarensis and Candida nivariensis, which are closely related to and identified as Candida glabrata by phenotypic assays. A total of 137 recent clinical isolates of C. glabrata identified by phenotypic characteristics was tested with C. bracarensis and C. nivariensis species-specific peptide nucleic acid fluorescence in situ hybridization probes. Three of 137 (2.2%) isolates were positive with the C. bracarensis probe, whereas the control strain, but none of the clinical isolates, was positive with the C. nivariensis probe. D1/D2 sequencing confirmed the identification of the three isolates as representing C. bracarensis. Clinically, one C. bracarensis isolate was recovered from a presumed infection, a polymicrobial pelvic abscess in a patient with perforated diverticulitis. The other two isolates were recovered from two adult oncology patients who were only colonized. C. bracarensis was white on CHROMagar Candida, had variable API-20C patterns that overlapped with C. nivariensis and some C. glabrata isolates, and had variable results with a rapid trehalose assay. Interestingly, an isolate from one of the colonized oncology patients was resistant to fluconazole, itraconazole, voriconazole, and posaconazole in vitro. In summary, C. bracarensis was detected among clinical isolates of C. glabrata, while C. nivariensis was not. One C. bracarensis isolate causing a presumed deep infection was recovered, and another isolate was azole resistant. Whether clinical laboratories should identify C. bracarensis will require more data.
机译：分子分类学研究表明，在表型划分的物种中有新的 Candida 物种，最好的例子是 Candida dubliniensis 。本研究旨在确定两种新的分子定义物种，即 bracarensis 和 candida nivariensis ，它们与 gladata密切相关并被鉴定为。表型分析。总共137个近期的 C临床分离株。用 C测试通过表型特征鉴定的glabrata 。 bracarensis 和 C。 nivariensis 物种特异性肽核酸荧光原位杂交探针。 137株分离株中有3株（2.2％）的C呈阳性。 bracarensis 探针，而 C则为对照菌株，但没有临床分离株阳性。 nivariensis 探针。 D1 / D2测序证实鉴定出三个分离株代表 C。 bracarensis 。临床上，一个 C。从疑似感染性穿孔性憩室炎患者的多菌性盆腔脓肿中获得了bracarensis 分离株。从仅定居的两名成年肿瘤患者中回收了其他两个分离株。 C。 Bracarensis 在CHROMagar念珠菌上呈白色，具有可变的API-20C模式，与 C重叠。 nivariensis 和一些 C。 glabrata 分离株，并通过快速海藻糖测定获得了可变结果。有趣的是，从一位定植的肿瘤患者中分离出的一种抗氟康唑，伊曲康唑，伏立康唑和泊沙康唑在体外具有耐药性。总之， C。在 C的临床分离株中检测到了bracarensis 。 glabrata ，而 C。 nivariensis 不是。一个 C。恢复了造成推测的深层感染的bracarensis 分离株，另一株对唑类耐药。临床实验室是否应识别 C。 bracarensis 将需要更多数据。

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