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首页> 外文期刊>Journal of Clinical Microbiology >Long-Term Stability at ?20°C of Aspergillus Galactomannan in Serum and Bronchoalveolar Lavage Specimens
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Long-Term Stability at ?20°C of Aspergillus Galactomannan in Serum and Bronchoalveolar Lavage Specimens

机译：曲霉半乳甘露聚糖在血清和支气管肺泡灌洗标本中于20°C的长期稳定性

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摘要

Research to develop and validate novel methods for diagnosis of aspergillosis based on detection of galactomannan requires the use of clinical specimens that have been stored frozen. Data indicating that galactomannan remains stable when frozen are scant. The objective of this study was to determine the stability of galactomannan in clinical specimens stored at ?20°C that were positive in the Platelia Aspergillus enzyme immunoassay when initially tested. Prospective real-time testing of serum and bronchoalveolar lavage (BAL) fluid pools from positive and negative patient specimens showed no decline in galactomannan index (GMI) over 11 months at ?20°C and no development of positive reactions in the negative-control pool. Retrospective testing of positive specimens that had been stored at ?20°C for 5 years showed that 28 of 30 serum (n = 15) or BAL (n = 15) specimens remained positive. These findings support the use of frozen serum or BAL specimens stored for at least 5 years in evaluation of diagnostic tests based on detection of galactomannan.
机译：为了研究和验证基于半乳甘露聚糖检测曲霉病的新方法，需要使用已冷冻保存的临床标本。指示半乳甘露聚糖在冷冻时保持稳定的数据很少。这项研究的目的是确定半乳甘露聚糖在？20°C储存的临床标本中的稳定性，该标本在最初测试时在白曲霉酶免疫测定中呈阳性。对来自阳性和阴性患者标本的血清和支气管肺泡灌洗液（BAL）池进行的前瞻性实时测试显示，在20°C的温度下，半乳甘露聚糖指数（GMI）在11个月内没有下降，并且阴性对照池中没有阳性反应的发展。对在20°C下保存5年的阳性标本进行的回顾性测试显示，在30个血清（ n = 15）或BAL（ n = 15）样品中有28个保持积极。这些发现支持使用存储了至少5年的冷冻血清或BAL标本来评估基于半乳甘露聚糖检测的诊断测试。

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