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首页> 外文期刊>Journal of Clinical Microbiology >Implementation of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry in Routine Clinical Laboratories Improves Identification of Coagulase-Negative Staphylococci and Reveals the Pathogenic Role of Staphylococcus lugdunensis
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Implementation of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry in Routine Clinical Laboratories Improves Identification of Coagulase-Negative Staphylococci and Reveals the Pathogenic Role of Staphylococcus lugdunensis

机译：常规临床实验室中基质辅助激光解吸电离-飞行时间质谱仪的实施改进了凝血酶阴性葡萄球菌的鉴定，并揭示了卢格顿葡萄球菌的致病作用

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The use of matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) for staphylococcal identification is now considered routine in laboratories compared with the conventional phenotypical methods previously used. We verified its microbiological relevance for identifying the main species of coagulase-negative staphylococci (CoNS) by randomly selecting 50 isolates. From 1 January 2007 to 31 August 2008, 12,479 staphylococci were isolated with phenotypic methods, of which 4,594 were identified as Staphylococcus aureus and 7,885 were coagulase negative staphylococci. Using MALDI-TOF MS from 1 January 2011 to 31 August 2012, 14,913 staphylococci were identified, with 5,066 as S. aureus and 9,847 as CoNS. MALDI-TOF MS allowed the identification of approximately 85% of the CoNS strains, whereas only 14% of the CoNS strains were identified to the species level with phenotypic methods because they were often considered contaminants. Furthermore, the use of MALDI-TOF MS revealed the occurrence of recently characterized Staphylococcus species, such as S. pettenkoferi, S. condimenti, and S. piscifermentans. Microbiological relevance analysis further revealed that some species displayed a high rate of microbiological significance, i.e., 40% of the S. lugdunensis strains included in the analysis were associated with infection risk. This retrospective microbiological study confirms the role of MALDI-TOF MS in clinical settings for the identification of staphylococci with clinical consequences. The species distribution reveals the occurrence of the recently identified species S. pettenkoferi and putative virulent species, including S. lugdunensis.
机译：与以前使用的常规表型方法相比，现在实验室认为使用基质辅助激光解吸电离-飞行时间质谱（MALDI-TOF MS）进行葡萄球菌鉴定。我们验证了其微生物学相关性，可通过随机选择50个分离株来鉴定凝固酶阴性葡萄球菌（CoNS）的主要种类。从2007年1月1日至2008年8月31日，采用表型方法分离出12,479株葡萄球菌，其中4,594例为金黄色葡萄球菌，7,885例为凝固酶阴性葡萄球菌。从2011年1月1日至2012年8月31日使用MALDI-TOF MS鉴定出14,913葡萄球菌，其中金黄色葡萄球菌为5,066，CoNS为9,847。 MALDI-TOF MS可以鉴定出约85％的CoNS菌株，而通过表型方法只能鉴定出14％的CoNS菌株至物种水平，因为它们通常被认为是污染物。此外，MALDI-TOF MS的使用揭示了最近鉴定的葡萄球菌种类的出现，例如S. pettenkoferi，S。condimenti和S. piscifermentans。微生物相关性分析进一步表明，某些物种显示出很高的微生物学意义，即，分析中所含的沙门氏菌40％与感染风险相关。这项回顾性微生物学研究证实了MALDI-TOF MS在临床环境中鉴定葡萄球菌的临床作用。物种分布揭示了最近鉴定出的S. pettenkoferi物种和推定的强毒物种，包括卢格链霉菌。

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