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首页> 外文期刊>Journal of Clinical Microbiology >Towards Development of Improved Serodiagnostics for Tularemia by Use of Francisella tularensis Proteome Microarrays
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Towards Development of Improved Serodiagnostics for Tularemia by Use of Francisella tularensis Proteome Microarrays

机译：通过使用土拉弗朗西斯菌蛋白质组芯片开发改进的Tularemia血清学诊断方法

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Tularemia in humans is caused mainly by two subspecies of the Gram-negative facultative anaerobe Francisella tularensis: F. tularensis subsp. tularensis (type A) and F. tularensis subsp. holarctica (type B). The current serological test for tularemia is based on agglutination of whole organisms, and the reactive antigens are not well understood. Previously, we profiled the antibody responses in type A and B tularemia cases in the United States using a proteome microarray of 1,741 different proteins derived from the type A strain Schu S4. Fifteen dominant antigens able to detect antibodies to both types of infection were identified, although these were not validated in a different immunoassay format. Since type A and B subspecies are closely related, we hypothesized that Schu S4 antigens would also have utility for diagnosing type B tularemia caused by strains from other geographic locations. To test this, we probed the Schu S4 array with sera from 241 type B tularemia cases in Spain. Despite there being no type A strains in Spain, we confirmed the responses against some of the same potential serodiagnostic antigens reported previously, as well as determined the responses against additional potential serodiagnostic antigens. Five potential serodiagnostic antigens were evaluated on immunostrips, and two of these (FTT1696/GroEL and FTT0975/conserved hypothetical protein) discriminated between the Spanish tularemia cases and healthy controls. We conclude that antigens from the type A strain Schu S4 are suitable for detection of antibodies from patients with type B F. tularensis infections and that these can be used for the diagnosis of tularemia in a deployable format, such as the immunostrip.
机译：人类的Tularemia主要由革兰氏阴性兼性厌氧性弗朗西斯菌tularensis的两个亚种引起：F. tularensis亚种。 tularensis（A型）和F. tularensis亚种。 holarctica（B型）。当前的Tularemia血清学检测是基于整个生物体的凝集，并且对反应性抗原的了解还不够。以前，我们使用蛋白质组学芯片，分析了来自A型菌株Schu S4的1,741种不同蛋白质，在美国的A型和B型Tularemia病例中分析了抗体反应。鉴定了十五种能够检测针对两种感染的抗体的显性抗原，尽管未以不同的免疫测定形式对其进行验证。由于A型和B型亚种密切相关，我们假设Schu S4抗原也可用于诊断由其他地理位置的菌株引起的B型图莱姆病。为了测试这一点，我们用西班牙241例B型Tularemia病例中的血清检测了Schu S4阵列。尽管西班牙没有A型毒株，但我们确认了对先前报道的某些相同的潜在血清诊断抗原的应答，并确定了对其他潜在的血清诊断抗原的应答。在免疫条上评估了五种潜在的血清诊断抗原，其中两种（FTT1696 / GroEL和FTT0975 /保守的假设蛋白）区分了西班牙tularemia病例和健康对照。我们得出的结论是，来自A型菌株Schu S4的抗原适用于检测来自B. tularensis感染患者的抗体，并且这些抗体可以以可展开形式（例如免疫条）用于诊断Tularemia。

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