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首页> 外文期刊>Journal of Clinical Microbiology >Use of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry To Identify Vancomycin-Resistant Enterococci and Investigate the Epidemiology of an Outbreak
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Use of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry To Identify Vancomycin-Resistant Enterococci and Investigate the Epidemiology of an Outbreak

机译：使用基质辅助激光解吸电离-飞行时间质谱法鉴定耐万古霉素的肠球菌并调查暴发流行病学

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摘要

The control of vancomycin-resistant enterococci (VRE) has become an increasing burden on health care resources since their discovery over 20 years ago. Current techniques employed for their detection include time-consuming and laborious phenotypic methods or molecular methods requiring costly equipment and consumables and highly trained staff. An accurate, rapid diagnostic test has the ability to greatly reduce the spread of this organism, which has the ability to colonize patients for long periods, potentially even lifelong. Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) is a technology with the ability to identify organisms in seconds and has shown promise in the identification of other forms of antimicrobial resistance in other organisms. Here we show that MALDI-TOF MS is capable of rapidly and accurately identifying vanB-positive Enterococcus faecium VRE from susceptible isolates. Internal validation of the optimal model generated produced a sensitivity of 92.4% and a specificity of 85.2%. Prospective validation results, following incorporation into the routine laboratory work flow, demonstrated a greater sensitivity and specificity at 96.7% and 98.1%, respectively. In addition, the utilization of MALDI-TOF MS to determine the relatedness of isolates contributing to an outbreak is also demonstrated.
机译：自从20多年前发现耐万古霉素肠球菌（VRE）以来，对保健资源的负担日益增加。用于检测它们的当前技术包括费时费力的表型方法或需要昂贵设备和消耗品以及训练有素的人员的分子方法。准确，快速的诊断测试能够大大减少这种生物的扩散，从而能够长期定居患者，甚至可能终生。基质辅助激光解吸电离飞行时间质谱仪（MALDI-TOF MS）是一种能够在几秒钟内识别出生物的技术，并且在鉴定其他生物的其他形式的抗药性方面显示出了希望。在这里，我们显示MALDI-TOF MS能够快速，准确地从易感菌株中鉴定 vanB 阳性粪肠球菌VRE。生成的最佳模型的内部验证产生了92.4％的灵敏度和85.2％的特异性。纳入常规实验室工作流程后，预期的验证结果显示出更高的灵敏度和特异性，分别为96.7％和98.1％。此外，还证明了利用MALDI-TOF MS来确定导致暴发的分离株的相关性。

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