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首页> 外文期刊>Journal of Clinical Microbiology >Molecular and Serological Diversity of Neisseria meningitidis Carrier Strains Isolated from Italian Students Aged 14 to 22 Years
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Molecular and Serological Diversity of Neisseria meningitidis Carrier Strains Isolated from Italian Students Aged 14 to 22 Years

机译：分离自意大利14至22岁学生的脑膜炎奈瑟氏球菌携带者菌株的分子和血清学多样性

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Neisseria meningitidis is an obligate human commensal that commonly colonizes the oropharyngeal mucosa. Carriage is age dependent and very common in young adults. The relationships between carriage and invasive disease are not completely understood. In this work, we performed a longitudinal carrier study in adolescents and young adults (173 subjects). Overall, 32 subjects (18.5%) had results that were positive for meningococcal carriage in at least one visit (average monthly carriage rate, 12.1%). Only five subjects tested positive at all four visits. All meningococcal isolates were characterized by molecular and serological techniques. Multilocus sequence typing, PorA typing, and sequencing of the 4CMenB vaccine antigens were used to assess strain diversity. The majority of positive subjects were colonized by capsule null (34.4%) and capsular group B strains (28.1%), accounting for 23.5% and 29.4% of the total number of isolates, respectively. The fHbp and nhba genes were present in all isolates, while the nadA gene was present in 5% of the isolates. The genetic variability of the 4CMenB vaccine antigens in this collection was relatively high compared with that of other disease-causing strain panels. Indications about the persistence of the carriage state were limited to the time span of the study. All strains isolated from the same subject were identical or cumulated minor changes over time. The expression levels and antigenicities of the 4CMenB vaccine antigens in each strain were analyzed by the meningococcal antigen typing system (MATS), which revealed that expression can change over time in the same individual. Future analysis of antigen variability and expression in carrier strains after the introduction of the MenB vaccine will allow for a definition of its impact on nasopharyngeal/oropharyngeal carriage.
机译：脑膜炎奈瑟氏球菌是通常在口咽粘膜上定殖的专性人类共鸣。运输是取决于年龄的，在年轻人中非常普遍。尚未完全理解马车与侵袭性疾病之间的关系。在这项工作中，我们对青少年和年轻人（173名受试者）进行了纵向携带者研究。总体而言，有32名受试者（18.5％）的结果在至少一次访视中对脑膜炎球菌携带呈阳性（平均每月携带率为12.1％）。在所有四次访问中，只有五名受试者测试为阳性。所有脑膜炎球菌分离株均通过分子和血清学技术鉴定。使用多基因座序列分型，PorA分型和4CMenB疫苗抗原的测序来评估菌株多样性。绝大部分阳性受试者被无效胶囊（34.4％）和荚膜B组菌株（28.1％）定殖，分别占分离株总数的23.5％和29.4％。在所有分离物中都存在 fHbp 和 nhba 基因，而在5％的分离物中存在 nadA 基因。与其他致病菌株相比，该收集物中4CMenB疫苗抗原的遗传变异性相对较高。关于马车状态持续性的指示仅限于研究的时间跨度。从同一对象中分离出的所有菌株随时间推移都是相同的或累积的微小变化。通过脑膜炎球菌抗原分型系统（MATS）分析了每个菌株中4CMenB疫苗抗原的表达水平和抗原性，这表明同一个人的表达会随时间变化。引入MenB疫苗后，对抗原变异性和携带株中表达的未来分析将有助于确定其对鼻咽/口咽运输的影响。

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