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首页> 外文期刊>Journal of Clinical Microbiology >Copy Numbers of Telomeric Repeat Sequences of Human Herpesvirus 6B in Clinical Isolates: Possibility of Mixed Infections
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Copy Numbers of Telomeric Repeat Sequences of Human Herpesvirus 6B in Clinical Isolates: Possibility of Mixed Infections

机译：人疱疹病毒6B在临床分离物中的端粒重复序列的拷贝数：混合感染的可能性

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In order to determine whether mixed infections of human herpesvirus 6B (HHV-6B) occur in immunocompetent and immunocompromised individuals, we examined the copy numbers of telomeric repeat sequences (TRS) of clinical isolates. In clinical isolates obtained from patients with exanthem subitum caused by primary HHV-6B infection, PCR products with HHV-6B TRS ranging between 400 and 800 bp were amplified. PCR products of various sizes were amplified in four clinical isolates from drug-induced hypersensitivity syndrome (DIHS) patients and 15 isolates from hematopoietic stem cell transplant (HSCT) recipients with HHV-6B reactivation. Based on the sequence analysis of the PCR products, the copy numbers of TRS in DIHS and HSCT patients were between 42 and 82 and 22 and >90, respectively. For two of the HSCT recipients, HHV-6B TRS PCR products of different sizes were detected in several isolates from each patient, which suggests mixed HHV-6B infections. In two of the posttransplant HHV-6B encephalitis patients, the sizes of the TRS nested PCR products amplified from the reactivated virus detected in the central nervous system differed from those of the virus detected in initial isolates from peripheral blood mononuclear cells. Taken together, these results suggest that PCR analysis of TRS copy number is a reliable tool for the discrimination of HHV-6B clinical isolates. Additionally, mixed HHV-6B infections occurred in HSCT recipients, and in some cases, compartmentalization of the HHV-6B strains to the central nervous system versus the blood compartment occurred in posttransplant HHV-6B encephalitis patients.
机译：为了确定在免疫能力强和免疫功能低下的个体中是否发生人疱疹病毒6B（HHV-6B）的混合感染，我们检查了临床分离株端粒重复序列（TRS）的拷贝数。在从原发性HHV-6B感染引起的散发性亚散的患者中获得的临床分离株中，扩增了HHV-6B TRS在400至800 bp之间的PCR产物。在来自药物诱导的超敏综合症（DIHS）患者的四个临床分离株和来自具有HHV-6B激活作用的造血干细胞移植（HSCT）受体的15个分离株中扩增了各种大小的PCR产物。根据PCR产物的序列分析，DIHS和HSCT患者的TRS拷贝数分别介于42和82之间以及22和> 90之间。对于两名HSCT接受者，在每位患者的数种分离物中检测到不同大小的HHV-6B TRS PCR产物，这表明存在混合HHV-6B感染。在两名移植后HHV-6B脑炎患者中，从在中枢神经系统中检测到的再活化病毒扩增的TRS嵌套PCR产物的大小与在从外周血单核细胞的最初分离物中检测到的病毒的大小不同。综上所述，这些结果表明TRS拷贝数的PCR分析是鉴别HHV-6B临床分离株的可靠工具。此外，在HSCT接受者中发生了混合的HHV-6B感染，在某些情况下，在移植后的HHV-6B脑炎患者中，HHV-6B菌株与中枢神经系统的分隔相对于血液分隔发生了。

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