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首页> 外文期刊>Journal of Clinical Microbiology >Predictive Value of Molecular Drug Resistance Testing of Mycobacterium tuberculosis Isolates in Valle del Cauca, Colombia
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Predictive Value of Molecular Drug Resistance Testing of Mycobacterium tuberculosis Isolates in Valle del Cauca, Colombia

机译：在哥伦比亚瓦莱德尔考卡的结核分枝杆菌分离株的分子耐药性检测的预测价值

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Previous evaluations of the molecular GenoType tests have promoted their use to detect resistance to first- and second-line antituberculosis drugs in different geographical regions. However, there are known geographic variations in the mutations associated with drug resistance in Mycobacterium tuberculosis, and especially in South America, there is a paucity of information regarding the frequencies and types of mutations associated with resistance to first- and second-line antituberculosis drugs. We therefore evaluated the performance of the GenoType kits in this region by testing 228 M. tuberculosis isolates in Colombia, including 134 resistant and 94 pansusceptible strains. Overall, the sensitivity and specificity of the GenoType MTBDRplus test ranged from 92 to 96% and 97 to 100%, respectively; the agreement index was optimal (Cohen's kappa, >0.8). The sensitivity of the GenoType MTBDRsl test ranged from 84 to 100% and the specificity from 88 to 100%. The most common mutations were katG S315T1, rpoB S531L, embB M306V, gyrA D94G, and rrs A1401G. Our results reflect the utility of the GenoType tests in Colombia; however, as some discordance still exists between the conventional and molecular approaches in resistance testing, we adhere to the recommendation that the GenoType tests serve as early guides for therapy, followed by phenotypic drug susceptibility testing for all cases.
机译：分子GenoType测试的先前评估促进了它们在不同地理区域中对一线和二线抗结核药物耐药性检测的用途。然而，在结核分枝杆菌中，与耐药性有关的突变存在已知的地理变异，尤其是在南美洲，关于与对一线和二线抗结核药物耐药相关的突变的频率和类型的信息很少。因此，我们通过在哥伦比亚测试了228株结核分枝杆菌菌株，包括134株耐药菌株和94株易感菌株，评估了GenoType试剂盒在该地区的性能。总体而言，GenoType MTBDR plus 测试的敏感性和特异性分别为92％至96％和97％至100％。一致性指数是最佳的（科恩卡帕值> 0.8）。 GenoType MTBDR sl 测试的灵敏度范围为84至100％，特异性为88至100％。最常见的突变是 katG S315T1， rpoB S531L， embB M306V， gyrA D94G和 rrs A1401G。我们的结果反映了GenoType测试在哥伦比亚的实用性;但是，由于在耐药性测试的常规方法和分子方法之间仍然存在一些矛盾，因此我们坚持建议GenoType测试作为早期治疗指南，然后对所有病例进行表型药物敏感性测试。

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