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首页> 外文期刊>Journal of Clinical Microbiology >Comparative Evaluation of Sensititre YeastOne and CLSI M38-A2 Reference Method for Antifungal Susceptibility Testing of Aspergillus spp. against Echinocandins
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Comparative Evaluation of Sensititre YeastOne and CLSI M38-A2 Reference Method for Antifungal Susceptibility Testing of Aspergillus spp. against Echinocandins

机译：曲霉属抗真菌药敏试验的敏感性YeastOne和CLSI M38-A2参考方法的比较评价。对抗棘球and素

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Aspergillus isolates with reduced susceptibility to current antifungal agents have been increasingly reported, highlighting the importance of antifungal susceptibility testing in clinical microbiology laboratory routine (1). In vitro reduced susceptibility of Aspergillus fumigatus isolates to echinocandins can be developed via FKS-dependent and FKS-independent mechanisms (2). Although clinical resistance of Aspergillus to echinocandins is limited (3), breakthrough infections in patients treated with echinocandins has been reported (4), and non-wild-type clinical isolates with minimal effective concentrations (MECs) above the epidemiological cutoff value have been found (5). Sensititre YeastOne (YO) is an adapted susceptibility system of the microbroth CLSI method based on the M27-A3 standard for yeasts, which uses alamarBlue as a colorimetric indicator. Sensititre YeastOne has been extensively evaluated for yeasts. There are fewer studies for molds, demonstrating high levels of agreement with the reference methodology for amphotericin B and azoles with Aspergillus spp. (6–12), while limited data exist for echinocandins. Since Sensititre YeastOne is widely used in routine microbiology laboratories, the aim of the present study was to assess its performance for in vitro susceptibility testing of echinocandins against the three most commonly isolated Aspergillus spp.—A. fumigatus, A. flavus, and A. terreus—compared to the reference Clinical and Laboratory Standards Institute (CLSI) broth microdilution (BMD) method.
机译：对目前的抗真菌药敏感性降低的曲霉菌分离株的报道越来越多，突出了在临床微生物学实验室常规中抗真菌药敏试验的重要性（1）。可以通过FKS依赖性和FKS依赖性机制在体外降低烟曲霉分离株对棘球and素的敏感性（2）。尽管曲霉菌对棘球and菌素的临床耐药性有限（3），但已报道了用棘枝and菌素治疗的患者出现突破性感染（4），并且已发现最低有效浓度（MEC）高于流行病学临界值的非野生型临床分离株（5）。 Sensititre YeastOne（YO）是基于酵母M27-A3标准的微泡CLSI方法改编的药敏系统，使用alamarBlue作为比色指示剂。 Sensititre YeastOne已被广泛评估酵母。霉菌的研究较少，这表明与两性霉素B和唑类曲霉菌的参考方法学高度吻合。 （6-12），而棘球and素的数据有限。由于Sensititre YeastOne被广泛用于常规微生物实验室中，因此本研究的目的是评估其对三种最常分离的曲霉菌（Apergillus spp.-A）棘球oc素的体外药敏试验的性能。与参考的临床和实验室标准协会（CLSI）肉汤微量稀释（BMD）方法相比，烟熏曲霉，黄曲霉和土曲霉。

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