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首页> 外文期刊>Journal of cell biology >Cellular interaction between fixed and living cells. Transfer of radioactive materials from living cells to fixed cells.
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Cellular interaction between fixed and living cells. Transfer of radioactive materials from living cells to fixed cells.

机译：固定细胞与活细胞之间的细胞相互作用。放射性物质从活细胞转移到固定细胞。

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Transfer of radioactive materials to fixed cells from an overlying layer of living cells has been examined to determine whether fixed cells can act as acceptors of glycosyltransferases of living cells. After the incubation of living cells were removed by EDTA treatment, and the radioactivity associated with the fixed cells was determined. Lipids, proteins and carbohydrates were found to be transfered from the living cells to the fixed cells. The amount of radioactivity transferred to the fixed cells was dependent on the number of both fixed and living cells and increased with the time of incubation. When fixed cells were treated with chloroform-methanol before the addition of living cells, the transfer of both lipids and proteins to the fixed cells decreased drastically, but only a slight decrease incarbohydrate transfer was observed. Most of the radioactive materials transferred from living cells labeled with glucosamine or fucose to chloroform-methanol-treated fixed cells were solubilized by trypsin but not by the detergents tested. Approximately 55% of the materials transferred from the cells labeled with glucosamine could be solubilized by hyaluronidase and chondroitinase, and the rest was solubilized by neuraminidase and a glycosidase mixture. The treatment of chloroform-methanol-extracted fixed cells with trypsin caused a significant decrease in the transfer from cells labeled with glucosamine. When nucleotide sugars were used as the radioactive precursor, no significant amount of radioactivity was transferred to the fixed cells.
机译：已经检查了放射性物质从活细胞上层转移到固定细胞的过程，以确定固定细胞是否可以充当活细胞糖基转移酶的受体。温育后，通过EDTA处理除去活细胞，并测定与固定细胞相关的放射活性。发现脂质，蛋白质和碳水化合物从活细胞转移到固定细胞。转移到固定细胞的放射性量取决于固定细胞和活细胞的数量，并且随着孵育时间的增加而增加。当在加入活细胞之前用氯仿-甲醇处理固定细胞时，脂质和蛋白质向固定细胞的转移都急剧减少，但仅观察到碳水化合物转移的轻微减少。从用葡糖胺或岩藻糖标记的活细胞转移到氯仿-甲醇处理的固定细胞中的大多数放射性物质是通过胰蛋白酶而不是通过测试的去污剂溶解的。从透明质酸酶和软骨素酶可溶解从葡萄糖胺标记的细胞转移的物质中的约55％，其余的则可通过神经氨酸酶和糖苷酶混合物来溶解。用胰蛋白酶处理从氯仿-甲醇中提取的固定细胞会导致葡萄糖胺标记的细胞的转移明显减少。当核苷酸糖用作放射性前体时，没有大量放射性转移到固定细胞上。

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